老年COPD患者血清SP-D、NE、IL-8和α-HBD水平的变化与病情进展的关系  

The Relationship of the Changes of Serum SP-D,NE,IL-8,α-HBDH Levels and Progress of the Disease in Elderly Patients with COPD

作　　者：王卓[1] 王滨[1] 洪侃[1] 万鹤鸣[1] 郁洁[1] 陆守荣[1] 

机构地区：[1]江苏省无锡市人民医院老年医学科,214023

出　　处：《放射免疫学杂志》2013年第5期552-555,共4页Journal of Radioimmanology

摘　　要：目的:探讨血清四项指标浓度的变化与慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)患者病情进展的关系和临床价值。方法:血清肺泡表面活性蛋白D(surfactant protein D,SP-D),中性粒细胞弹性蛋白酶(neutronphil elastase,NE)采用酶联免疫法(ELISA)检测,白细胞介素8(interleukin 8,IL-8)含量则采用放射免疫分析(RIA),α-羟丁酸脱氢酶(alpha-hydroxybutyric dehydrogenase,α-HBDH)测定采用全自动生化分析法(ACA)。结果:临床及测定结果:两组间除性别和平均年龄两项临床记录指标外,余五项指标均存在显著性差异(P<0.05,P<0.01)。血清指标中SP-D水平Ⅰ级组较正常对照组略升高,但无显著性差异(P>0.05),Ⅱ、Ⅲ级组水平则均显著高于正常对照组(P<0.05,P<0.0),患者分级组间比较也存在显著性差异(P<0.05)。IL-8水平则于Ⅰ级组已显著高于正常对照组(P<0.05),Ⅱ、Ⅲ级组水平则均显著高于正常对照组(P均<0.01),患者分级组间比较也存在显著性差异(P均<0.05)。NE水平的变化统计结果同IL-8。α-HBDH活性的变化Ⅰ、Ⅱ级两组较正常对照组皆无显著统计学差异(P均>0.05),Ⅲ级组水平则显著高于Ⅱ级组和正常对照组(P<0.05,P<0.01)。相关分析结果:血清指标与患者呼吸功能指标FEV1的相关性分析显示,FEV1与SP-D水平Ⅰ级组无显著相关性(r=-0.26,P>0.05),Ⅱ级组呈弱相关(r=-0.36,P=0.05),Ⅲ级组则呈显著负相关(r=-0.76,P<0.05)。FEV1与IL-8水平Ⅰ级组呈显著负相关(r=-0.55,P<0.05),Ⅱ级组呈显著负相关(r=-0.65,P<0.05),Ⅲ级组则呈显著负相关(r=-0.70,P<0.05)。FEV1与NE水平Ⅰ级组呈显著正相关(r=-0.60,P<0.05),Ⅱ级组呈显著负相关(r=-0.65,P<0.05),Ⅲ级组则呈显著负相关(r=-0.70,P<0.05)。FEV1与α-HBDH水平Ⅰ级组无显著相关性(r=-0.30,P>0.05),Ⅱ级组也无显著相关(r=-0.15,P>0.05),Ⅲ级组则呈弱相关(r=-0.41,P=0.05)。结论:血清四项指标浓度的变化与COPD患者病情进展的关系密切;其Objective To explore the relationship and the clinical significance of changes of four serum markers （SP-D, NE, IL- 8,α-HBD） in elderly patients with COPD. Methods Serum SP-D, NE levels were measured with ELISA, while IL-8 with RIA,α- HBDH with ACA. Results Clinical and measurement results： the cases and control appeared to be well matched on age and gender （ as shown in table 1 ）, however, there proved significant statistical differences among five indicators（ P 〈0.05 ,P 〈0.01 ）. Serum lev- el of SP-D in Ⅰ group showed slightly higher than that of control group, but there expressed no significant statistical difference（ P 〉 0.05） ,Those in Ⅱ and Ⅲ group presented sirnificanfly higher than normal control group（P 〈 0.05 ,P 〈 0.01 ）, there were also signifi- cant differences between those in patients with grade group（P 〈 0.05 ）. Serum level of IL-8 in Ⅰ group indicated sitmificantly higher than normal control group（P 〈0.05） ,those in Ⅱ and Ⅲ group proved markedly higher than the normal control group（P 〈0.01, P 〈 0.01 ） ,there were also sit nificant differences between those in patients with grade group（ P 〈0.05）. Serum level of NE in statistical results with IL-8. Serum levels ofα-HBDH in Ⅰ group and Ⅱ group proved no statistically nificant differences（P〉0.05）, those in Ⅲ group ffained significontly hlot, her thanⅡgroup and normal control group（P 〈0.05 ,P 〈0.01 ）. Correlatire analysis results： analysis of serum nmrkers and respiratory function parameters FEV1 showed that： FEV1 and serum level of SP-D no signifieant correlatian in Ⅰ group（r= -0.26 ,P〉0.05）, but slightly correlatlon in II gronp （r= -0.36,P =0.05）, while signifieantly negative correlation in Ⅲgnmp（r = -0. 76,P 〈0.05）. FEV1 and serum level of IL-8 were sinificantly negative correlation in Ⅰ, Ⅱ, Ⅲ group（r= -0.55,P 〈0.05; r = -0.65,P 〈0.05; r= -0.70,P 〈0.05, respectively）. FEV1 and serum level Of NE were significandy pos

关 键 词：慢性阻塞性肺疾病肺泡表面活性蛋白D 中性粒细胞弹性蛋白酶 白细胞介素8 α-羟丁酸 脱氢酶 

分 类 号：R725.6[医药卫生—儿科]