光泽精流动注射化学发光法测定呋喃西林的含量  

Determination of nitrofurazone by flow-injection chemiluminescence( FI-CL) with lucigenin-hydrogen peroxide system

作　　者：叶瑞洪[1] 林谦[1] 卢贵文[1] 吴琳[1] 

机构地区：[1]福建师范大学福清分校化学系,福建福清350300

出　　处：《宁德师范学院学报（自然科学版）》2013年第3期240-244,共5页Journal of Ningde Normal University(Natural Science)

基　　金：福建省教育厅A类科技项目(JA11283);食品安全分析与检测教育部重点实验室(福州大学)开放课题项目(FS1108);福建省大学生创新实验项目(20421134)

摘　　要：基于光泽精在碱性条件下可以被过氧化氢氧化产生化学发光，结合流动注射技术建立了一种直接测定呋喃西林的流动注射化学发光新方法．该方法的最佳测定条件：H2O2的浓度为0．020mol／L，NaOH的最佳浓度为0．080mol／L，光泽精浓度为1．0×10^7mol／L，负高压为800V，蠕动泵主泵运行10s后副泵运行30s，接着主泵继续转动10s，副泵30s，如此循环．线性范围0．0100～0．100pug／mL和1．00～10．0p,g／mL，检出限为0．00800p,g／mL，对0．100μg／mL的呋喃西林连续进行110C平行测定，其相对标准偏差为3．70％，该法已成功测定了三种不同浓度呋喃西林精粉的含量，其相对标准偏差分别为3．03％、1．60％、2．04％，平均回收率在95．8％-101．2％之间．Since the lucigenin in the alkaline conditions can be oxidized by the hydrogen peroxide chemiluminescence, combined with a flow injection technique, a flow injection analysis with direct ;determination method of nitrofurazone luminescence was established. The optimum measurement conditions for the method： the concentration of H2O2 was 0.020mol/L, the optimal concentration of NaOH was 0.080mol/L, the lucigenin concentration was 1.0×10^-7mol/L, the negative pressure is 800V; the peristaltic pump ran at 10s, the hind auxiliary pump ran at 30s, then the main pump continued to rotate at 10s, and the auxiliary pump ran at 30s, so did the cycle. The linear range was of 0.0100~ 0.100lxg/mL and 1.00~10.01xg/mL, the detection limit is 0.00800~g/mL, 11 parallel determinations performed continuously for 0.1001xg/mL furacilin, and the relative standard deviation was 3.70%. The method has successfully determined the contents of three different kinds of furacilin powder, the relative standard deviation were 3.03%, 1.60%, and 2.04%, respectively and the average recovery rates were 95.8%-101.2%.

关 键 词：化学发光 流动注射 呋喃西林 光泽精一过氧化氢 

分 类 号：O657.3[理学—分析化学]