人乳头瘤病毒16型晚期基因L1的原核表达质粒的构建和鉴定  

Construction and identification of late gene L1 of human Papillomavirus type 16

作　　者：郭淑元[1] 凌虹[1] 钟照华[1] 庄敏[1] 林道红[1] 曲章义[1] 郭彩玲[1] 谷鸿喜[1] 

机构地区：[1]哈尔滨医科大学微生物学教研室,黑龙江哈尔滨150086

出　　处：《中国地方病学杂志》2000年第6期472-473,共2页Chinese Jouranl of Endemiology

基　　金：黑龙江省科委重点攻关项目!(G98-CO80 3)

摘　　要：目的　构建人类乳头瘤病毒 (HPV) 16型晚期基因 L1的原核表达质粒 ,以期从中选出能够高效表达 HPV16 L1的重组子 ,为进一步表达 L1蛋白奠定基础。方法　应用定向克隆策略 ,将 HPV16 L1基因分别克隆到原核表达载体 p Trac99A和 p ET- 2 1c中。结果　通过酶切鉴定选出了重组子 p Trc99A- HPV16 L1和 p ET- 2 1c-HPV16 L1。结论　 HPV16Objective To construct prokaryotic expression plasmid of Human Papillomavirus type 16(HPV16)L1 gene from which we may select recombinant that can express HPV16 L1 in a high level,and which may make basis for gene expression of L1.Methods The L1 gene of HPV16 Chinese strain is cloned into prokaryotic expression vector pTrc99A and pET 21c by means of determined direction strategy.Results Recombinant plasmid,pTrc99A HPV16L1 and pET 21c HPV16L1,was screened by digestion of restrict endonuclease.Conclusions The construction of prokaryotic expression plasmid of HPV16L1 was achieved.

关 键 词：人类乳头瘤病毒16 晚期基因L1 基因重组 质粒 

分 类 号：R373.9[医药卫生—病原生物学] R394[医药卫生—基础医学]