猪源高产蛋白酶芽胞杆菌的分离与鉴定  

Isolation and Identification of High Proteinase-producing from Pig Feces

作　　者：李三栋[1] 丁轲[1,2] 罗伟光[1] 彭春平[1] 孙二刚 刘永垒 

机构地区：[1]河南科技大学宏翔发酵饲料实验室,河南洛阳471003 [2]河南宏翔生物科技有限公司,河南汝州467500

出　　处：《饲料博览》2013年第9期34-37,共4页Feed Review

基　　金：河南省教育厅科学技术研究重点项目(13B230987);河南科技大学SRTP项目(20122244)

摘　　要：利用酪蛋白培养基从猪粪便样品中分离产蛋白酶的芽胞杆菌,采用福林酚法测定其酶活力,对筛选到的高酶活菌株进行形态学、生理生化和分子鉴定。结果表明,从86株分离菌中筛选了一株酶活较大的菌株B.A464,革兰氏阳性菌,呈粗杆状,能形成卵圆形芽孢,16S rDNA序列长度是1 463 bp,与蜡样芽孢杆菌的同源性高达99%,鉴定为蜡样芽孢杆菌,所产蛋白酶活力为2.19×103U·mL-1。Bacillus producing proteinase were isolated from pig feces by medium containing casein as sub- strate, the proteinase activities of above bacteria were detected by Forint phenol method, and the high proteinase- producing Bacillus were screened. They were identified by the colony morphology, microscopic morphology in com- bination with the physiological and biochemical feature and 16S rDNA sequence. The results showed that the strain B.A464 with the highest proteinase activity of 2.19×10^3U·mL^-1, was obtained, and it was gram strain positive, short rod, forming oval spores, and had the ability of fermenting many sugars. Based on the 16S rDNA sequence homology, comparative analysis showed that it belonged to the genus bacillus and most closery related to bacillus cereus strain 08001 with 99% sequence simmarity. So the strain B.A464 was identified to be bacillus cereus.

关 键 词：蛋白酶 芽孢杆菌 16S RDNA 分离 鉴定 

分 类 号：S828[农业科学—畜牧学] S816.8[农业科学—畜牧兽医]