不同外植体类型诱导岩黄连愈伤组织和再分化的初步研究  

Preliminary study on callus induced and redifferentiation of Corydalis saxicola Bunting by different explants

作　　者：苏江[1] 岑忠用[1] 邓晰朝[1] 覃勇荣[1] 

机构地区：[1]河池学院化学与生命科学系,广西宜州546300

出　　处：《广东农业科学》2013年第17期13-15,F0002,共4页Guangdong Agricultural Sciences

基　　金：广西教育厅科研项目(200911MS221);河池学院引进人才科研启动费项目(2010QS-N008);广西高校重点实验室桂西北特色资源研究与开发实验室项目(桂教科研[2006]6号)

摘　　要：为探讨不同外植体对岩黄连在组织培养中愈伤组织诱导和植株再生的影响,从而筛选出较适合岩黄连组织培养的外植体材料,为建立岩黄连无性繁殖体系提供参考。以岩黄连的茎尖、茎节(带侧芽)、叶柄、叶片为试验材料,采用不同方式进行消毒,并接种于不同配方培养基上进行培养。结果表明:岩黄连不同类型外植体在诱导愈伤组织和植株再生上有较大的差异,叶柄和叶片的愈伤组织诱导率明显高于茎节和茎尖,其中叶片诱导率最高、为46.67%,而茎尖愈伤组织的再分化率最高、为66.7%。叶片为岩黄连诱导愈伤组织较好的外植体材料,茎尖较适合于诱导岩黄连植株再生。In order to find out the influence of different explants on callus induced and redifferentiation in tissue culture of Corydalis saxicola Bunting , then to screen suitable explants for tissue culture and provide reference for establishing asexual reproduction system of C. saxicola Bunting, this experiment used different disinfection treatments and explants culturing on different medium, stem-tip, internodes （with lateral buds）, petioles and leaves of C.saxicola Bunting were used as materials. The results showed that there was significant differences in callus induced and plant regeneration by different explants of C.saxicola Bunting. The callus induced rate of petioles and leaves were higher than that of stem node and stern-tip. The callus induced rate of leaves was the highest with 46.67%, while redifferentiation rate of stem-tip callus was the highest with 66.7%. The leaf was favorable explants for callus induction while stem-tip was favorable explants for plant regeneration of C. saxicola Bunting in tissue culture.

关 键 词：岩黄连 愈伤组织 诱导 再分化 

分 类 号：Q949.748.1[生物学—植物学]