灰葡萄孢菌BCRho3基因的生物信息学分析  

Bioinformatics analysis of Botrytis cinerea BCRho3 gene

作　　者：董德成[1] 易萌[1] 张传博[1] 

机构地区：[1]贵州师范大学生命科学学院,贵州贵阳550001

出　　处：《广东农业科学》2013年第17期146-149,157,共5页Guangdong Agricultural Sciences

基　　金：贵州省星火计划项目(黔科合农字[2012]5056号)

摘　　要：利用生物信息学方法,对灰霉病菌中BCRho3基因的理化性质、亲疏水性、信号肽、亚细胞定位、结构及功能等进行预测研究,同时构建BCRho3同源基因的系统发育树,为下一步功能研究提供参考。结果表明,BCRho3基因编码产物为亲水性蛋白,不具有信号肽和跨膜结构,二级结构以α-螺旋和无规则卷曲为主,主要在细胞质中发挥生物学作用。序列分析表明,BCRho3基因编码产物可能作为生长因子来发挥作用,同时参与转录及转录调控过程,可能在产孢等生长发育过程中具有调节作用,因此可能在灰霉病的致病性中起关键作用。Rho3基因编码产物同源性及进化树表明,灰霉病菌BCRho3基因编码产物与核盘菌、杨盘二孢菌等菌种的Rho3基因编码产物具有高度的同源性,遗传距离较近。This research used bioinformatical methods to predict the physical-chemical characteristics, hydropathy and hydrophobicity, signal peptide, subcellular localization, structure and function of BCRho3 gene from Botrytis cinerea, and constructed phylogenetic tree of Rho3, all of this would provide further support for following research. The result shows that BCRho3 is a hydrophilic protein which has not signal peptide and transmembrane structure, its secondary structure is primarily composed of alpha helix and random coil and it performs biological function mainly in the cytoplasm. Sequence analysis indicates that this protein might functions as growth factor, and may involve in transcription regulation and transcription, so it may play important role during growth and development such as spore production, thus it is likely to play a key role in pathogenicity of B.cinerea. The homology and evolutionary tree of Rho3 shows that BCRho3 and related proteins of Sclerotinia sclerotiorttm and Marssorrina brurmea are close in the phylogenetic tree and identity, which shows highly homology.

关 键 词：BCRho3基因 生物信息学 结构与功能 

分 类 号：Q75[生物学—分子生物学]