一株极端嗜盐多孢放线菌新种的分类研究  

A novel exteremely Halophilic actinomycete of Actinopolysporais determined using apolyphasic approach

作　　者：关统伟[1,2] 赵辉平[1] 张利莉[2] 车振明[1] 

机构地区：[1]西华大学生物工程学院,四川成都610039 [2]塔里木大学,新疆阿拉尔625000

出　　处：《西北师范大学学报（自然科学版）》2013年第5期97-102,共6页Journal of Northwest Normal University(Natural Science)

基　　金：国家自然科学基金资助项目(31060003);教育部春晖计划项目(Z2012022);教育部科技重点项目(209145);西华大学重点项目(Z1220530)

摘　　要：通过多相分类技术证明一株分离自中国西北部新疆盐湖环境的放线菌新种XHU139．采用微生物的表型、生理生化特征、细胞化学及其分子遗传学手段研究放线菌新种XHUl39的系统分类地位．16SrRNA基因序列系统发育分析表明：菌株XHU139在多孢放线菌菌属中形成一个独立的分支单元，同近缘物种Actinopolyspora alba、Actinopolysporax iniiangensis和Actinopolyspora erythraea的同源性分别为97．6％、97．6％和97．1％，与它们的DNA—DNA杂交值分别为36．4％、31．3％和26．1％，因此被考虑作为多孢放线菌属的一个新成员．菌株XHUl39的最适生长温度是35℃，最适生长pH7．0，最适生长盐浓度12％～1f{％；细胞水解糖为xylos、glucose、ribose和arabinose，主要的醌型是MK-9（H4）（49．8％）和MK-10（H4）（24．2％），主要的脂肪酸是iso—C16：0（28．0％）和anteiso—C17：o（27．6％），细胞磷脂是diphosphatidylglycerol、phosphatidylglycer01、phosphatidylcholine、phosphatidylinosit01和2个未知的磷脂类型．多相分类研究结果表明，菌株XHUl39为多孢放线菌属的一个新物种．The aim of this study is to identify the exact taxonomic status of the novel strain XHU139, which is isolated from a salt lake in Xinjiang Province, north-west China, by using a polyphasic taxonomy study. Its taxonomic status is determined on the basis of the phenotypic, physiological, chemotaxonomic and genotypic data of strain XHU139. Phylogenetic analysis ba＇~ed on an almost-complete 16S rRNA gene sequence of the strain show that it formed a well-seperated sub-branch within the radiation of the genus Actinopolyspora and the organism is most closely related to Actinopolyspora alba （97.6%）, Actinopolyspora xinjiangensis （97.6%） and Actinopolyspora erythraea （97.1%）. However, it has relatively lower mean values for DNA-DNA relatedness with the above strains（36.4 %）, 31.3% and 26.1%, respectively）. Optimal growth occurre at 35 ℃, pH 7.0 and in the presence of 12% -13% NaC1. The whole-cell sugar pattern consists of xylose, glucose, ribose and arabinose. The major fatty acids are iso-C16· 0 （28.0%）and anteiso-C17 ： 0 （27.6%）. The diagnostic phospholipid detected is diphosphatidylglycerol, phosphatidylglycerol phosphatidylcholine phosphatidylinositol and two holipids. The predominant menaquinone are MK 9 （ H4 ） （49.8%） and MK 10 （ ttl ） G＋C content of the genomic DNA is 66.4 mol%. On the basis of the above phenotypic, hemotaxonomic and genotypic data, we consider strain XHU139 to represent a novel nus Actinopolyspora.

关 键 词：盐湖 嗜盐放线菌 新物种 多相分类 

分 类 号：Q93-331[生物学—微生物学]