基于Fe_3O_4@Au磁性纳米粒子修饰丝网印刷电极的微囊藻毒素免疫传感器研究  

An Immunosensor for Microcystins Based on Fe_3O_4@Au Magnetic Nanoparticle Modified Screen-Printed Electrode

作　　者：张金果[1] 康天放[1] 薛瑞[1] 孙雪[1] 

机构地区：[1]北京工业大学环境与能源工程学院,北京100124

出　　处：《分析化学》2013年第9期1353-1358,共6页Chinese Journal of Analytical Chemistry

基　　金：北京市教委科技发展基金重点项目(No.KZ201110005006);北京市自然科学基金(No.8102009);北京市人才强教深化计划-创新团队(No.PH R201007105);北京工业大学研究生科技基金资助

摘　　要：将核壳型Fe3O4@Au磁性纳米粒子修饰在丝网印刷工作电极表面,再通过纳米金和微囊藻毒素-(亮氨酸-精氨酸)抗体(anti-MCLR)之间的吸附作用,将抗体固定于电极表面,以牛血清白蛋白(BSA)封闭非特异性吸附位点,制得了检测MCLR的电流型免疫传感器。该传感器基于直接竞争的免疫分析模式,以辣根过氧化物酶偶联的微囊藻毒素(MCLR-HRP)为标记物,用差分脉冲伏安法检测微囊藻毒素,在优化的实验条件下,此免疫传感器响应的峰电流值与微囊藻毒素浓度在0.79～12.9μg/L范围内呈良好的线性关系,检测限为0.38μg/L。对实际水样进行了微囊藻毒素的加标回收实验,回收率在95%～107%之间。此免疫传感器具有测定速度快、灵敏高、携带方便等优点。The coreshell Fe3O4@Au magnetic nanoparticles were modified onto the screen-printed working electrode surface,and the antibody of microcystin-（leucine-arginine）（anti-MCLR） was immobilized on the modified electrode surface by the adsorption between Au nanoparticles and the antibody.Subsequently,bovine serum albumin（BSA） was used to block the non-specific adsorption sites of the immunosensor to obtain immunosensor for the detection of MCLR.The immunosensor was based on the direct competitive immunoassay format between the labeled agent of horseradish peroxidase-conjugated MCLR（MCLR-HRP） and the analyte.MCLR was determined by differential pulse voltammetry（DPV）.Under the optimal experimental conditions,the peak current response decreased proportionally with the concentration of MCLR in the range of 0.79-12.90 μg/L with a detection limit of 0.38 μg/L.The developed biosensor was used to determine MCLR in real water samples,and the recoveries of standard additions were in the range of 95% to 107%.The proposed immunosensor possesses the properties of fast,sensitive and portable,etc.

关 键 词：Fe3O4@Au磁性纳米粒子 微囊藻毒素 丝网印刷电极 免疫传感器 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]