基于~1HNMR的代谢组学方法对大鼠砒霜给药后血清的研究  

Studies on Serum of Arsenic Trioxide Treated Rats by ~1H NMR Based Metabonomics

作　　者：张盼盼[1,2] 章文军[1] 薛蓉[2] 李晓晶[2] 闫威[3] 裴奉奎[2] 

机构地区：[1]河北工业大学化工学院,天津300130 [2]中国科学院长春应用化学研究所,长春130022 [3]长春中医药大学附属医院,长春130021

出　　处：《分析化学》2013年第9期1434-1438,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20975097)资助项目

摘　　要：运用代谢组学方法研究了Wistar大鼠灌胃给药砒霜2和10 mg/kg剂量血清代谢的变化。通过核磁共振技术检测大鼠血清的代谢指纹图谱,然后利用主成分分析法分析空白组和砒霜给药组的代谢物差异,研究不同剂量砒霜在大鼠体内的急性生物效应。结果表明,砒霜对大鼠的急性靶向器官是肝脏,随着剂量增大,毒性增强;低剂量给药导致氧化应激,高剂量给药诱导细胞凋亡;低和高剂量给药均出现糖、氨基酸及脂质代谢紊乱,且能量代谢异常。The metabolites of rats′ serum caused by arsenic trioxide intragastric infusion（dosed at 2,10 mg/kg body weight,respectively） were investigated through metabonomics.The metabolic profiling detected by NMR subjected to principal component analysis（PCA）,which find out the different metabolites of the control group and arsenic trioxide treated group,to explore the acute biological effects.The results revealed that arsenic trioxide caused acute damage on rats′ liver,and the toxicity effect increased with the increase of dose.Low dosage administration arsenic trioxide gave rise to reactive oxygen species（ROS）,and high dosage administration arsenic trioxide induced cell apoptosis.Sugar,amino acid fat metabolism disorder and energy metabolism abnormality occurred in both low and high dosed rats.

关 键 词：代谢组学 砒霜 核磁共振 血清 主成分分析 

分 类 号：R96[医药卫生—药理学]