基于噬菌体展示抗体的电化学发光免疫传感器检测相思子毒素研究  

Determination of Abrin by Electrochemiluminescence Immunosensor Based on Phage-displayed Antibody

作　　者：刘冰[1] 童朝阳[1] 刘威[1] 郝兰群[1] 穆晞惠[1] 黄启斌[1] 

机构地区：[1]防化研究院,国民核生化灾害防护国家重点实验室,北京102205

出　　处：《分析化学》2013年第9期1449-1453,共5页Chinese Journal of Analytical Chemistry

基　　金：总装预研课题(No.404070205)资助项目

摘　　要：以多克隆抗体包被磁性微球制备捕获探针,以表面负载大量三联吡啶钌标记物的噬菌体展示抗体为发光探针有效放大信号,成功建立了相思子毒素电化学发光免疫传感检测方法。利用本方法检测相思子毒素,浓度在0.005～100μg/L范围内与电化学发光强度呈良好的对数线性关系,拟合方程为lgY=0.701lgX+1.767(R=0.9964,N=7,p<0.0001),检测限达0.005μg/L(S/N=3)。与采用单克隆抗体制备标记探针相比,检测灵敏度提高20倍,可用于相思子毒素的痕量检测。A new efficient immunoassay strategy for the determination of Abrin was developed.The assay consisted of a double antibody sandwich format in which immobilized polyclonal antibody（pcAb） was used for capture probe,and Ru（bpy）32＋ labeled phage-displayed antibody for electrochemiluminescence（ECL） probe to amplify the ECL signal.A linear relationship between ECL intensity and the concentration of Abrin in the range of 0.005-100 μg/L was obtained.The linear regression equation was lgY=0.701lgX＋1.767（R=0.9964,n=7,p0.0001） with a detection limit of 0.005 μg/L（S/N=3）.In comparison with the ECL probe based on monoclonal antibody（mcAb）,this method gave a 20-fold better sensitivity in Abrin detection.Due to the low detection limit,this method holds great promise in toxin trace detection.

关 键 词：噬菌体展示抗体 相思子毒素 电化学发光 免疫传感器 

分 类 号：Q946.1[生物学—植物学]