玉米叶片蛋白对AM菌根接种和(或)砷胁迫的应答  

Response of Maize Leaf Proteins Induced/Modulated by AM Mycorrhizal Inoculation and (or) Arsenic Stress

作　　者：王真辉[1] 袁坤[1] 杨礼富[1] 张俊伶[2] 

机构地区：[1]中国热带农业科学院橡胶研究所/农业部儋州热带作物科学观测实验站,海南儋州571737 [2]中国农业大学资源环境学院/农业部植物营养重点实验室/教育部植物-土壤互作重点实验室,北京100193

出　　处：《中国农业科学》2013年第18期3758-3767,共10页Scientia Agricultura Sinica

基　　金：国家自然科学基金项目(30571105;31071872)

摘　　要：【目的】研究接种菌根与砷胁迫条件下玉米叶片组织相关蛋白质的变化。【方法】以玉米植株叶片为材料,采用双向凝胶电泳技术(2-DE)研究玉米叶片蛋白表达谱对丛枝菌根(AM菌根)接种和(或)砷胁迫的应答。【结果】经软件分析并搜索NCBInr数据库,结果显示,砷胁迫植株叶片中有7个蛋白点被成功鉴定,其中有3个未知蛋白,其余4个分别为2-phosphoglycerate kinase、oxidoreductase、MAP3K delta-1 protein kinase和Os06g0262800。接种且加砷处理植株叶片中有11个蛋白点被成功鉴定,有4个未知蛋白,其余蛋白分别为oxygen-evolving enhancer protein 3-1、putative M protein、SNF1-related protein kinase 2.2、ATP synthase CF1 beta subunit、ATP synthase CF1 alpha subunit、pathogenesis-related protein 10、MAP kinase kinase和MEI1 protein。【结论】菌根接种促进加砷处理玉米植株生长,并显著降低玉米植株地下部砷浓度。玉米植株叶片蛋白表达量及种类的变化,表明砷胁迫条件下菌根接种有可能激发与植物生长、养分吸收以及抗性有关的蛋白产生应答,有助于提高玉米对砷毒的抗性。[Objective] In this paper, proteomics were performed to analyze the changes in leaf proteins of maize in response to As additions and mycorrhizal inoculation. [ Method ] The leaf proteome of maize was analyzed using two-dimensional electrophoresis and mass spectrometry. A targeted proteomic approach was applied to determine the putative identity of some proteins induced/modulated by arsenic （As） and/or mycorrhizal inoculation. [Result] The proteomics aimed at comparing the leaf protein profiles of maize growing in substrate amended with arsenic or unamended. Seven differentially expressed protein spots were identified. Three of them were identified as unknown protein and the remaining four were 2-phosphoglycerate kinase, oxidoreductase, MAP3K delta-1 protein kinase and Os06g0262800. Another comparasion of leaf proteins between plants with arsenic stress and those with As stress plus mycorrhizal inoculation revealed that 22 spots were differentially displayed in response to mycorrhizal inoculation and 11 differentially expressed protein spots were identified. Four of them were identified as unknown protein and the other seven were oxygen-evolving enhancer protein 3-1, putative M protein, SNF 1-related protein kinase 2.2, ATP synthase CF 1 beta subunit, ATP synthase CF1 alpha subunit, pathogenesis-related protein 10, MAP kinase kinase and MEI1 protein, respectively. [ Conclusion] The dry weights of maize plants with As addition were improved by mycorrhizal innoculations, root As concentrations were also decreased significantly. Changes in the amount and types of leaf protein expression in maize plants indicated that mycorrhizal inoculation might simulate the proteins associated with plant growth, nutrient uptake and resistance to produce responses under arsenic stress conditions. Therefore, it＇s helpful to improve the resistance of maize plants to arsenic toxicity.

关 键 词：丛枝菌根真菌 玉米 比较蛋白质组学 砷胁迫 

分 类 号：S513[农业科学—作物学]