父源性印记基因在牛早期胚胎中的表达  

The Expression of Paternal Imprinting Genes in Bovine Early Stage Embryo

作　　者：张春强[1] 赵德超[1] 韩志玲[1] 张文广[1] 张家新[1] 

机构地区：[1]内蒙古农业大学动物科学学院/动物遗传育种与繁殖自治区重点实验室,呼和浩特010018

出　　处：《中国农业科学》2013年第18期3887-3893,共7页Scientia Agricultura Sinica

基　　金：国家科技支撑计划(2011BAD19B01);内蒙古自然科学基金项目(2010MS0407)

摘　　要：【目的】研究父源印记基因在牛早期胚胎中的表达模式。【方法】利用Real-time PCR技术,检测Gnas、Grb10和Xist这3个父源性印记基因在牛体外受精(In vitro Fertilization,IVF)和孤雌激活(Parthenogenetic Activation,PA)胚胎各发育阶段的表达情况。【结果】对于IVF和PA两种来源的牛胚胎,Gnas在胚胎各发育阶段的表达没有明显差异;Grb10在2细胞阶段的PA胚胎中的表达量明显高于在IVF胚胎的,但从4细胞阶段到囊胚阶段,Grb10在两种来源的牛胚胎中表达没有明显差异;从2细胞阶段到8细胞阶段,Xist在两种来源的牛胚胎中表达没有明显差异,但从16细胞期开始,Xist在PA胚胎中的表达明显高于IVF胚胎的表达量。【结论】对于牛IVF胚胎和PA胚胎,Gnas、Grb10和Xist从2细胞期到囊胚期都可以检测到表达,但表达模式不同。这3个父源性印记基因在牛胚胎发育过程中可能发挥着重要的作用。[Objective] The objective of the experiment is to investigate the expression pattern of paternal imprinting genes in bovine early stage embryo. [Method] The expression profile of paternal imprinting genes Gnas, GrblO, and Xist were detected in bovine embryo derived from in vitro fertilization （IVF） and parthenogenetic activation （PA） by real-time PCR. [Result] There were no difference in imprinted expression profile of Gnas between IVF and PA erabryo. GrblO expression was significantly higher during 2-cell stage in PA embryo than that of IVF embryo, however, no difference was observed in expression of GrblO between IVF and PA embryos from 4-cell satge to blastcyst stage. Xist expression was similar between PA and IVF embryo from 2-cell to 8-cell stage. But in the later stage, Xist expression was higher significantly in PA embryo than that in IVF embryo. [Conclusion] Gnas, GrblO and Xist were expressed differently in bovine IVF embryo and PA embryo from 2-cell stage to the blastocyst stage, and they might play an important role in bovine embryo development.

关 键 词：牛 父源性印记基因 早期胚胎 表达 

分 类 号：S823[农业科学—畜牧学]