辛伐他汀与阿托伐他汀对高糖培养大鼠肾小球系膜细胞p27蛋白表达的影响  

Effect of simvastatin and atorvastatin on the expression of p27 protein in mesangial cells of rat under high glucose

作　　者：徐艳丽[1] 李青菊[1] 

机构地区：[1]郑州大学第二附属医院内分泌科,450014

出　　处：《重庆医学》2013年第28期3400-3402,共3页Chongqing medicine

摘　　要：目的探讨辛伐他汀和阿托伐他汀对高糖培养的大鼠肾小球系膜细胞p27表达的影响。方法将体外培养大鼠肾小球系膜细胞分为正常对照组(葡萄糖浓度5.6mmol/L)、高糖组(葡萄糖浓度30mmol/L)、高糖辛伐他汀组(葡萄糖浓度30mmol/L,辛伐他汀浓度10μmol/L)、高糖阿托伐他汀组(葡萄糖浓度30mmol/L,阿托伐他汀浓度10μmol/L)。每组设置6个复孔,于24、48、72、120h收集细胞,Western blotting法测定大鼠肾小球系膜细胞p27蛋白的表达,逆转录PCR测定p27mRNA的表达。结果高糖组p27蛋白浓度及mRNA水平均较正常对照组升高(P<0.05);高糖辛伐他汀组及高糖阿托伐他汀组p27蛋白浓度及mRNA水平均较高糖组降低(P<0.05)。结论他汀类药物通过调节肾小球系膜细胞p27的表达,可发挥非依赖降脂的肾脏保护作用。Objective To explore the effect of simvastatin and atorvastatin on the level of p27 protein in mesangial cells of rat under high glucose. Methods Cultured rat mesangial cells in vitro were divided into four groups：normal glucose（5.6 mmol/L glu- cose）,high glucose（30 mmol/L glucose）,high glucose（30 mmol/L glucose） plus simvastatin（10 μmol/L）,high glucose（30mmol/L glucose） plus atorvastatin（10 μmol/L）. After treatrnent for 24,48,72 120 hours, the cells were collected. The protein level of p27 was detected by Western blotting and the mRNA level of p27 was detected by RT-PCR. Results Compared to the normal glucose group,the levels of p27 protein and mRNA in the high glucose group had a significant increase（P〈0.05）. Compared to the high glucose group,the level of p27 protein and mRNA in the high glucose plus simvastatin or atorvastatin group both had a significant decrease（P〈0.05）. Conclusion By modulating the expression of P27 in mesangial cells,statins could become the independent lipid- decreasing drug to prevent kidney.

关 键 词：辛伐他汀 阿托伐他汀 高血糖 大鼠肾小球系膜细胞 P27蛋白 

分 类 号：R587.2[医药卫生—内分泌] R692.9[医药卫生—内科学]