右美托咪定预处理对急性肺损伤大鼠的保护作用  

The protective effect of dexmedetomidine preconditioning on acute lung injury in rats

作　　者：葛东建[1] 祁宾[1] 李金玉[1] 

机构地区：[1]南京医科大学附属淮安第一医院麻醉科,江苏淮安223300

出　　处：《重庆医学》2013年第28期3405-3407,共3页Chongqing medicine

摘　　要：目的探讨Akt信号通路在右美托咪定预处理对抗脂多糖(LPS)致急性肺损伤(ALI)中的作用。方法成年SD大鼠24只,随机分为对照组(T0组)、LPS注射后1h(T1组)、3h(T2组)和6h组(T3组),检测肺组织中Akt和p-Akt的含量。大鼠54只随机分为对照组(C组)、ALI 6h组(ALI组)和右美托咪定+ALI 6h组(D+ALI组),检测p-Akt表达、肺泡灌洗液(BALF)蛋白浓度、细胞凋亡指数,观察肺组织病理变化。大鼠40只随机分为ALI组和D+ALI组,观察48h生存率。结果与T0组比较,T1、T2和T3组p-Akt表达依次减少(P<0.05)。与ALI组比较,D+ALI组p-Akt表达明显升高(P<0.05),但仍低于C组(P<0.05),BALF蛋白浓度,细胞凋亡指数均降低(P<0.05),但仍高于C组(P<0.05);D+ALI组病理损伤较ALI组减轻。D+ALI组48h生存率明显高于ALI组(P<0.05)。结论右美托咪定可能通过增加ALI组织中p-Akt的浓度发挥保护作用。Objective To investigate the role of Akt signaling in the protective effect of dexmedetomidine preconditioning on the lipopolysaccharide（LPS）-indueed acute lung injury（ALI） in rat. Methods Twenty-two SD rats were randomly divided into 4 groups：before LPS injection group（T0 group）,1 h after LPS injection group（T1 group）,3 h after LPS injection group（T2 group） and 6 h after LPS injection group（T3 group） ,the expression of Akt and p-Akt in the process of LPS-induced ALI was investigated. Another 54 rats were divided into 3 groups：control group（C group）, 6 hours of ALI group（ALI group） and Dexmedetomidine＋ ALl group（D＋ ALI group）, the expression of p-Akt in lung tissues, the protein concentration in bronchoalveolar lavage fluid （BALF）, apoptotic index and the pathological changes in the lung tissues were observed. Forty rats were divided into ALI group and ALI＋ D group to investigate the 48 h-survival ratio. Results Compared with TO group,the level of p-Akt expression in T1,T2, T3 groups were deceased in a time-dependent manner（P〈0.05）. Compared with ALI group, p-Akt in D ＋ ALI group increased significantly（P〈0.05） but was still lower than that in C group（P〈0.05） ;the protein concentration in BALF and the apoptotic in- dex in D ＋ALI groups were significantly lower than those in ALI group（P〈 0.05）, but still higher than those in C group （P〈 0.05）. The 48 h survival ratio was significantly decreased in D＋ ALI group comparing with ALI group（P〈0.05）. Conclusion Dexmedetomidine preconditioning might display protective effect via activating p-Akt signaling pathway in LPS-induced ALI.

关 键 词：右美托咪定 急性肺损伤 脂多糖类 P-AKT 

分 类 号：R614[医药卫生—麻醉学]