表皮生长因子与碱性成纤维生长因子对人牙髓干细胞增殖的影响  

Effects of EGF and bFGF on proliferation of human dental pulp stem cells

作　　者：徐皑[1] 吴桂堂 刘兴容[1] 

机构地区：[1]泸州医学院附属口腔医院口腔内科,四川泸州646000 [2]江苏省徐州市口腔医院牙体牙髓科

出　　处：《泸州医学院学报》2013年第4期356-359,共4页Journal of Luzhou Medical College

摘　　要：目的:探讨表皮生长因子(epidermal growth factor,EGF)和碱性成纤维生长因子(basic fibroblast growth factor,bFGF)对人牙髓干细胞(human dental pulp stem cells,hDPSCs)增殖能力的影响。方法:采用噻唑蓝(MTT)比色法分别测定不同浓度EGF和bFGF在不同时间,单独或联合应用对hDPSCs增殖能力的影响。结果:50 ng/ml EGF和20 ng/ml bFGF促hDPSCs增殖能力最强。以50ng/ml EGF、20ng/ml bFGF单独或联合作用,其促hDPSCs增殖能力均具有时间依赖性,第3 d、5 d、7 d,EGF、bFGF促hDPSCs增殖作用显著增强,二种生长因子联合作用,促增殖作用显著高于其单独作用效果,差异具有统计学意义(P<0.05)。结论:生长因子EGF和bFGF最大效应浓度分别为:50 ng/ml、20 ng/ml。EGF和bFGF单独作用均能显著促进hDPSCs的增殖,二者联合作用对hDPSCs的增殖有一定的协同作用。Objective： To investigate the effects of epidermal growth factor （EGF） and basic fibroblast growth factor（bFGF）on the proliferation of human dental pulp stem cells（hDPSCs） in vitro. Methods： Colorimetric assay was used to measure the dose - and time - depedent effects of EGF and bFGF on the proliferation of hDPSCs. Results：50 ng/ml EGF and 20 ng/ml bFGF had the strongeste effect on promoting the proliferation of hDPSCs re-spectively. The effect of 50 ng/ml EGF and 20 ng/ml bFGF, whether alone or combined was time-depedent. It was significantly stronger at 3, 5, 7 d （P 〈 0.05）, and the combined usege of the two cytokines was obviously stronger than used alone（P〈0.05）.Conclusion： The optimal concentration of EGF and bFGF is 50 ng/ml and 20 ng/ml respectively. Both EGF and bFGF can significantly promote the proliferation of hDPSCs. The combination use of two growth factors has a certain synergism.

关 键 词：牙髓干细胞 表皮生长因子 碱性成纤维生长因子 增殖 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]