西农萨能羊脂肪酸合酶(FASN)基因启动子序列的克隆与序列分析  

Cloning and Sequence Analysis of FASN Gene Promoter from Xinong Saanen Goat

作　　者：王海滨 韩雪峰[2] 祁昱[2] 罗军[2] 

机构地区：[1]贵阳市农业委员会,贵州贵阳550081 [2]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《山地农业生物学报》2013年第4期333-337,共5页Journal of Mountain Agriculture and Biology

基　　金：公益性行业(农业)科研专项项目(201103038)

摘　　要：以陕西省千阳县种羊场的足月正常分娩、健康的西农萨能羊为试验材料,运用PCR方法对其FASN基因启动子部分序列进行了克隆和序列分析,获得了西农萨能羊FASN基因启动子序列片段719 bp(GenBank收录号EF556550.1)。序列分析显示,TATA盒位于-35 bp处,1个类似于CAAT盒的序列位于-68 bp处,这些都是典型的真核生物启动子元件,TATA盒上游区域有76%以上的G/C含量和4个GC盒(GGGCGG和CCGCCC),潜在的核转录因子结合位点Sp1位于-99 bp、-174 bp、-255 bp和-320 bp。Using normal delivery and healthy Xinong Saanen goat from Qianyang Goat Seed Farm of Shanxi Province as experimental materials, the partial sequence of FASN gene promoter region was cloned and analyzed in the present work. Fragment length of FASN gene promoter cloned from Xinong Saanen goat was 719 bp （ GenBank accession no. EF556550.1 ）. TATA box was located in - 35 bp, and a similar CAAT box was located in -68 bp, both of which were the typical eukaryotic promoter elements. The upstream domain of TATA box contained 76% G/C and four GC boxes （ GGGCGG and CCGCCC）, and potential binding sites of nuclear transcription factor Spl was at positions - 99 bp, - 174 bp, - 255 bp and - 320 bp.

关 键 词：山羊 脂肪酸合酶基因 启动子 

分 类 号：Q751[生物学—分子生物学]