两种免疫学检验方法对于结核病的临床价值比较  

Comparison of clinical value between two immunological methods for tuberculosis

作　　者：黄胜[1] 余继英[1] 成波[1] 周丹[1] 雷治国[1] 陈林[1] 邹宇洁[2] 

机构地区：[1]松滋市人民医院,湖北松滋434200 [2]武汉大学第一临床学院,湖北武汉430060

出　　处：《临床肺科杂志》2013年第11期2051-2052,共2页Journal of Clinical Pulmonary Medicine

摘　　要：目的探讨蛋白芯片法与胶体金法两种方法检测结核抗体对结核病的临床诊断价值。方法 91例分为活动性结核和非结核两组,活动性结核组分为肺结核组和结核性胸腹膜炎组。均采用两种方法测结核抗体,两种方法对照比较。结果两种检测方法在肺结核组中有显著差异,P<0.005;蛋白芯片法和胶体金法在肺结核中的阳性率分别为72.3%、44.7%,在结核性胸腹膜炎中的阳性率分别为42.1%、26.3%;蛋白芯片法在结核组中总的阳性率为63.6%,胶体金法总的阳性率为39.4%,联合两种方法检测灵敏度71.2%,特异度48.0%,假阴性率28.8%,假阳性率62.0%,约登指数0.192,符合率64.8%。结论蛋白芯片法对活动性肺结核尤其是涂阴肺结核诊断有一定参考价值;胶体金法诊断结核价值有限,特别是单一方法检测时价值较小。Objective To discuss the clinical value of protein chips and colloidal gold in the detection of tuberculosis. Methods 91 patients were divided into the active and non-active tuberculosis groups, and the active group were further divided into the TB group and the tuberculosis peritonitis and pleuritis group. All patients were given the above two methods to detect tuberculosis antibody. Results The positive rate of TB detection by the two methods was respectively 72. 3% and 44. 7% in the TB group, and 42. 1% and 26.3% in the tuberculosis peritonitis and pleuritis group. The total positive rate of protein chips was 63.6%, and colloidal gold 39.4% in the TB group. The sensitivity of the two combined methods was 71.2%. The specificity was 48%. The false negative rate was 28.8%. The false positive rate was 62%. The Youden index was 0. 192. The rate of consistence was 64. 8%. Conclusion Protein chips method is effec- tive in the diagnosis of TB especially the negative sputum smear ones, while colloidal gold is limited in the diagnosis of TB.

关 键 词：结核 蛋白芯片法 胶体金法 诊断 

分 类 号：R446.6[医药卫生—诊断学] R52[医药卫生—临床医学]