普通菜豆抗白粉病基因SCAR标记鉴定  

Identification of Powdery Mildew Resistant Genes based on SCAR Markers in Common Bean ( Phaseolus vulgaris L. )

作　　者：吴星波[1,2] 郝俊杰[1] 张晓艳[1] 万述伟[1] 李红卫[1] 邵阳[1] 孙吉禄[1] 

机构地区：[1]青岛市农业科学研究院,山东青岛266100 [2]西南大学园艺园林学院,重庆400715

出　　处：《生物技术进展》2013年第5期357-362,共6页Current Biotechnology

基　　金：现代农业产业技术体系专项资金资助(CARS-09);农业部"引进国际先进农业科学技术"项目(2012Z-52:2011-G1(3)-08);青岛市农科院博士基金项目资助

摘　　要：利用8个来自于普通菜豆抗白粉病基因SCAR标记（SAU5、SS18、SF6Em3、SF12R9、SF13R10、SF18R7、SF18R15和SMelEm5）引物组，对78份普通莱豆资源进行抗白粉病分子标记的鉴定。结果表明：在这78份资源中，SF12R9、SF13R10、SFl8R7、SF18R15和SMelEm5均无扩增带。有52份资源含有SAU5标记，66份资源含有SF6Em3标记，76份资源含有SS18标记。76份资源含有2～3个标记。该研究明确了78份参试菜豆资源所含的抗白粉病基因类型，并筛选出抗自粉病基因聚合体的参试菜豆资源。Eight SCAR primer combinations （SAU5, SS18, SF6Em3, SF12R9, SF13R10, SF18R7, SF18R15 and SMelEmS） of common bean powdery mildew resistant genes were used to check the genome DNA of 78 common bean accessions. The SCAR primer pairs of SF12Rg, SF13R10, SF18RT, SF18R15 and SMelEm5 did not amplified target bands at all; SAU5 marker appeared in 52 accessions, SF6Em3 marker in （56 accessions, and SS18 marker in 76 accessions. 2-3 SCAR markers appeared in 76 accessions respectively. The types of powdery mildew resistant genes in each of the 78 accessions have been identified, and the accessions with pyramiding powdery mildew resistance genes were selected.

关 键 词：普通菜豆 SCAR 菜豆白粉病 抗病基因 分子标记 

分 类 号：S643.1[农业科学—蔬菜学]