大熊猫粪便菌群ERIC-PCR指纹图谱的分析及优势菌群的鉴定  

Analysis of characteristics of faecal flora pandas by ERIC-PCR fingerprinting and identification of the dominant flora

作　　者：崔明全[1] 何廷美[1,2] 钟志军[1] 王承东[2] 张婷婷[1] 周潇潇[1] 周紫峣[1] 李德生[2] 张和民[2] 李才武[2] 何永果[2] 彭广能[1] 

机构地区：[1]四川农业大学动物医学院/动物疫病与人类健康四川省重点实验室,四川雅安625014 [2]中国林业科学研究院大熊猫研究中心/四川雅安碧峰峡基地,四川雅安625007

出　　处：《畜牧与兽医》2013年第9期6-11,共6页Animal Husbandry & Veterinary Medicine

基　　金：国家自然基金项目(31272620);大熊猫国际合作资金项目(SD0632);教育部"长江学者和创新团队发展规划"创新团队项目(IRT0848)

摘　　要：探讨1只野生大熊猫和不同年龄段圈养大熊猫粪便菌群的多样性和相似性,并鉴定野生大熊猫圈养之后,其粪便中的优势菌群。对来自3个年龄段9只(3只亚成体、3只成年体、3只老年体)圈养大熊猫及1只野生大熊猫圈养前、后的11份粪便细菌的DNA进行ERIC-PCR指纹图谱分析,并利用16S rRNA基因序列分析对优势菌条带进行鉴定。结果显示:该只野生大熊猫粪便菌群多样性比圈养大熊猫丰富;圈养大熊猫粪便菌群多样性:成年体>亚成体>老年体;16s rRNA和ERIC-PCR指纹图谱优势条带鉴定的优势菌结果有差异性。结果表明:大熊猫的肠道菌群多样性易受生长环境、年龄因素的影响;其次,不同年龄段的圈养大熊猫,其肠道菌群的相似性与年龄因素不相关;利用ERIC-PCR指纹图谱优势条带鉴定优势菌的方法有一定的局限性。In the current study, the diversity and similarity of the gut were investigated for a wild giant panda and 9 captive giant pandas of different age groups, and the dominant bacteria of the intestinal flora was identified for the wild giant after the captivity. Fecal samples of captive pandas were collected from 3 subaduh, 3 adult and 3 senile pandas, and 1 wild panda before and after at Bifengxia zoological institutions in Ya＇an. Bacteria DNA was extracted from fecal bacteria and analyzed by ERIC-PCR. An advantage bacteria DNA band on the finger- print of the wild giant panda in captivity was purified, cloned, sequenced and alignmented. Then, the predominant bacteria were checked again via 16S rRNA method and compared with the results of identification of ERIC-PCR. The results revealed that the diversity of intestinal flora of the wild giant panda was more abundant than that of captive giant pandas and the diversity of feces intestinal flora of adult pandas group was most abundant than that of the other pandas in captive populations groups. In addition, the predominant flora results via ERIC- PCR fingerprinting and 16S rRNA method were not related, implyed that the ERIC-PCR fingerprinting method was limited. No significant similarity of the intestinal flora regarding to the ages factor was seen in all giant pandas. Our results indicated that the diversity of intestinal flora of the giant panda was susceptible to the growth environmental and age factors.

关 键 词：野生大熊猫 圈养大熊猫 肠道菌群 ERIC—PCR指纹图谱 16S RRNA基因 

分 类 号：S852.61[农业科学—基础兽医学]