41种中草药对3种鲟源病原菌的体外抑菌效果  

In vitroinhibitory effect of 41 Chinese herbs on three sturgeon-pathogenic bacteria

作　　者：杨移斌[1] 曹海鹏[1] 夏永涛[2,3] 杨婷[4] 赵蕾[1] 胡鲲[1] 杨先乐[1] 

机构地区：[1]上海海洋大学国家水生动物病原库,上海海洋大学农业部淡水水产种质资源重点实验室,上海市水产养殖工程技术研究中心,上海高校知识服务平台上海海样大学水产动物遗传育种中心(ZF1206),上海201306 [2]中国水产科学研究院,北京100039 [3]杭州千岛湖鲟龙科技股份有限公司,杭州310000 [4]山西医科大学第二临床医学院,太原030000

出　　处：《淡水渔业》2013年第4期80-84,共5页Freshwater Fisheries

基　　金：国家重大科技成果转化项目(ZD-2012-345);上海高校知识服务平台项目(ZF1206);公益性行业(农业)科研专项经费(201203085);国家863计划项目(2011AA10A216);国家自然科学基金资助项目(31172430);上海市科技兴农重点攻关项目(沪农科攻字(2011)第4-8号)

摘　　要：采用平板打孔法在观察了弗氏柠檬酸杆菌(Citrobacter freundii)、鲁氏耶尔森氏菌(Yersinia ruckeri)、嗜水气单胞菌(Aeromonas hydrophila)等3种鲟源病原菌对41种中草药敏感性的基础上,进一步采用二倍稀释法测定了具有良好抑菌活性的中草药对3种病原菌的最小抑菌浓度和最小杀菌浓度。实验结果表明,3种病原菌对乌梅、石榴皮、地榆、杞子等均高度敏感,对杜仲、柴胡、丹参、熟地黄等均中度敏感,对麦冬、当归、胖大海、玉竹均不敏感。此外,乌梅、石榴皮、地榆、杞子对3种病原菌的最小抑菌浓度和最小杀菌浓度分别为7.81 mg/mL和15.625 mg/mL、15.625 mg/mL和31.25 mg/mL、15.625 mg/mL和31.25 mg/mL以及15.625 mg/mL和31.25 mg/mL,而杜仲、柴胡、丹参、熟地黄等中度敏感中草药对3种病原菌的最小抑菌浓度和最小杀菌浓度均分别高于31.25 mg/mL和62.5 mg/mL。In the present study, the sensitivity of three pathogenic strains isolated from sturgeon, including Citrobacterfreundii, Yersinia Ruckeri, Aeromonas hydrophila against 41 species of Chinese herbs were observed, and based on the resuits of sensitivity, the minimum inhibitory concentration （MIC） and minimum bactericidal concentration （MBC） of theChinese herbs with good inhibitory activity to three bacterial pathogens were further determined. The experimental results showed that, the three bacterial pathogens were all highly susceptible to Prunus mume, Punica granatum, Sanguisorba offieinali and Barbary wolfberry fruit, intermediately susceptible to Eucommia ullmoides, Bupleurum chinense, Salvia mialtiorrhiza and Rehmannia glutinosa, and resistant to Ophiopogon japonicus, Angelica sinensis, Sterculia lychnophora and Polygonatum odoratum. In addition,the MICs and MBCs of P.mume,P.granatum,S.offieinali,B, wolfberry fruit were 7.81 mg/mL and 15.625 mg/mL, 15.625 mg/mL and 31.25 mg/mL, 15.625 mg/mL and 31.25 mg/mL, 15.625 mg/mL and 31.25 mg/mL, respectively. However, the MICs and MBCs of E. ullmoides, B.chinense, S.mialtiorrhiza, R.glutinosa were above 31.25 mg/ml and 62.5 mg/ml.

关 键 词：鲟(Acipenser sp.) 弗氏柠檬酸杆菌(Citrobacter freundii) 鲁氏耶尔森氏菌(Yersinia ruckeri) 嗜水气单胞菌(Aeromonas hydrophila) 中草药 抑菌效果 

分 类 号：S948[农业科学—水产养殖]