基于电渗驱动电磁阀切换恒电位检测的微全分析系统  

Micro Total Analysis System Based on Electroosmotic Drive Solenoid Valve Switch and Potentiostatic Detection

作　　者：张效伟[1] 

机构地区：[1]青岛理工大学理学院,山东青岛266033

出　　处：《仪表技术与传感器》2013年第8期65-68,共4页Instrument Technique and Sensor

基　　金：国家自然科学基金项目(20775039);青岛理工大学横向课题(Z-2012-275)

摘　　要：液流驱动和溶液切换是限制微全分析系统自动化的瓶颈,大多数光学检测系统体系庞大,无法实现系统的小型化和集成化。文中设计了基于电渗驱动电磁阀切换恒电位检测的便携式微全分析系统。该系统由可控高压电源、电渗驱动、电磁阀切换、微柱分离、在柱反应、恒电位检测及控制和数据采集单元组成,整个系统用自制接口卡和VC语言程序,通过计算机控制实现自动化操作。利用该系统实现了工业废水中大肠杆菌的高分辨、高灵敏检测,分离效率为7.8×104plates/m,检出限为93 cfu/mL,对相同的大肠杆菌样品,该系统测定的灵敏度比文中3篇相关文献提高8倍,分离效率提高3倍。结果表明:该系统设备紧凑、操作方便、灵敏度高,测定结果准确可靠,可用于实际样品的现场、实时分析。Solution drive and switch is the main restriction for automatization of micro total analysis system（μTAS）.Moreover,most of optical detection systems have bulky volumes,which make miniaturization and integration of the system very difficult.Consequently,a portable μTAS based on electroosmotic drive,solenoid valve switch and potentiostatic detection was designed.The system consisted of controllable high voltage,electroosmotic drive,solenoid valve switch,microcolumn separation,on-column reaction,potentiostatic detection and control and data collection module.The whole system was controlled by a computer with interface card and Visual C program.The highly resolving and sensitive analysis of E.coli in industrial waste water was realized with separation efficiency of 7.8×104 plates/m and detection limit of 93 cfu/mL.For the same E.coli sample,the sensitivity and separation efficiency with the proposed system were improved 8-fold and 3-fold compared with the instrumentation in three references of this paper.The results show that,the system has the advantages including compact structure,high sensitivity and convenience for in-situ,real-time analysis of actual samples.

关 键 词：电渗驱动 电磁阀切换 恒电位检测 

分 类 号：TH83[机械工程—仪器科学与技术]