COX-2抑制剂对膀胱癌细胞株裸鼠成瘤的影响  

Inhibitive effect of COX-2 on human bladder cancer cell xenografts in nude mice

作　　者：张军卫[1] 靳风烁[2] 龚晋迁[1] 彭东涛[1] 王刚[1] 程伟[1] 潘永军[1] 

机构地区：[1]重庆市第九人民医院泌尿外科,重庆400700 [2]第三军医大学大坪医院野战外科研究所泌尿外科,重庆400042

出　　处：《现代泌尿外科杂志》2013年第5期453-456,共4页Journal of Modern Urology

基　　金：重庆市卫生局面上项目(No.06-2-061)

摘　　要：目的探讨COX-2抑制剂对膀胱癌T24细胞株裸鼠成瘤性的影响。方法 BALB/c裸鼠27只分为3组,每只皮下接种膀胱癌T24细胞5×106活细胞数建立移植瘤动物模型。COX-2抑制剂药物干预组(吲哚美辛、塞来昔布)采用喂饲途径,吲哚美辛3mg/kg,塞来昔布10mg/kg;对照组给予生理盐水溶液药物的投给。30d后处死裸鼠,取瘤块称重,测量肿瘤体积,行免疫组化、半定量RT-PCR、Wester Blot检测移植瘤COX-2表达。结果对照组细胞接种裸鼠后第5天可见肿瘤长出,第7天各组均有肿瘤长出,第30天后用药组移植瘤生长较对照组明显减慢。免疫组化染色、RT-PCR、Western-blot结果均显示对照组COX-2表达明显,而药物干预组均少量表达。结论选择性与非选择性COX-2抑制剂在实验中表现出良好的抗肿瘤特性。ObjectiveTo establish the nude mice xenograft models of bladder cancer cell T24 and to study the inhibitive effects of Cyclooxygenase2 （COX2）. Methods Each of 27 athymic BALB/c mice was injected with 5×106 T24 bladder cancer cells. The mice were then divided equally into three groups. One group received oral indomethacin 3 mg/kg per mouse daily, the other 10 mg/kg celecoxib per mouse daily, and the control group received normal saline. The nude mice were killed after 30 days, and the weight and volume of xenografts were recorded. Histological hematoxylinosin staining and immunocytochemical SABC technique were employed to explore the histopathological changes. The expression of COX2 in the xenografts was detected by RTPCR and Westernblot. Results On the 5th day after transplantation, xenografts were found in the control group. On the 7th day, xenografts were observed in the other two groups. The expression level of COX2 reduced and the weight and volume of the xenografts decreased more in the groups treated with indomethacin or celecoxib.Conclusions Selective and nonselective COX2 inhibitors can inhibit the growth of xenografts in nude mice.

关 键 词：环氧化酶-2 膀胱肿瘤 塞来昔布 吲哚美辛 

分 类 号：R691.4[医药卫生—泌尿科学]