紫茉莉悬浮细胞培养体系的建立  

Studies on Establishment of Suspension Cell System for Mirabilis jalapa L.

作　　者：赵倩[1] 林景卫[1] 冯雅萍[1] 陈丽静[1] 马慧[1] 钟鸣[1] 

机构地区：[1]沈阳农业大学生物科学技术学院,辽宁省农业生物技术重点实验室,沈阳110866

出　　处：《热带亚热带植物学报》2013年第5期453-458,共6页Journal of Tropical and Subtropical Botany

基　　金：国家自然科学基金项目(31070448)资助

摘　　要：为建立紫茉莉（Mirabilis jalapa L.）悬浮细胞培养体系，以紫茉莉无菌苗叶片诱导的愈伤组织为材料，筛选紫茉莉悬浮细胞的适宜培养体系。结果表明，紫茉莉愈伤组织在MS＋2,4-D1mgL^-1＋KT0．5mgL^-1的液体培养基中悬浮继代培养3～4次，能得到稳定的悬浮细胞系。培养基的pH值为5．5～5．9，蔗糖浓度为30gL^-1更适合悬浮细胞的生长。紫茉莉悬浮细胞的生长曲线大致呈s型。最佳继代培养时间是10d，培养液的体积为40mL时，接种量为7．5mL，可以较好地保持悬浮细胞系。1L培养液中可提取分泌蛋白（0．42±0．15）g。这些有助于对悬浮细胞提取分泌蛋白的研究。In order to establish suspension cell system of Mirabilis jalapa L., the calli induced from leaves of aseptic seedlings of M. jalapa were used as material, the optimum suspension culture system was studied. The results showed that the stable suspension cell system could be obtained after calli were subcultured 3 - 4 times on MS medium with 2,4-D 1 mg·L^-1 and KT 0.5 mg·L ^-1. The optimum pH was 5.5 - 5.9 with 30 g L-1 sucrose. The growth curve of suspension cells of M. jalapa was S-type, and subculture time was about 10 days. The optimum inoculum was 7.5 mL in 40 mL medium. Secretory protein was （0.42 ± 0.15） g extracted from 1 L medium. These were useful for studying secretory protein extracted from suspension system.

关 键 词：紫茉莉 悬浮细胞 悬浮培养 分泌蛋白 

分 类 号：S685.16[农业科学—观赏园艺]