MiAsg分子克隆及与南方根结线虫病害的关系  

Molecular Clone of MiAsg Gene and the Its Relationship with Disease Caused by Meloidogyne incognita

作　　者：梅眉[1,2] 黄永红[2,3] 茆振川[2] 刘志敏[1,4] 谢丙炎[2] 

机构地区：[1]湖南农业大学园林园艺学院,长沙410128 [2]中国农业科学院蔬菜花卉研究所,北京100081 [3]农业部南亚热带果树生物学与遗传资源利用重点实验室,广东省农业科学院果树研究所,广州510640 [4]湖南第一师范学院,长沙410205

出　　处：《分子植物育种》2013年第5期585-591,共7页Molecular Plant Breeding

基　　金：国家重点基础发展计划项目(2009CB119000);公益性行业(农业)科研专项(201103018);国家自然科学基金重点项目(31030057);国家大宗蔬菜产业技术体系项目(CARS-25)共同资助

摘　　要：根结线虫(Meloidogyne spp.)是一种世界性的植物病害。在前期研究中,通过利用生物信息学方法在线虫全基因组中预测了一些功能基因。本研究以预测到的线粒体ATP合成酶g亚基基因(Asg)序列设计特异引物克隆了南方根结线虫(M.incognita)的Asg基因(MiAsg),对其序列进行了特征分析后,利用病毒诱导的基因沉默(VIGS)技术,将其导入番茄植株,然后接种M.incognita,研究MiAsg基因与根结线虫病害的关系。结果表明,克隆到的MiAsg基因与预测到的MiAsg基因相似性高达100%。接种M.incognita 60 d后,MiAsg基因沉默的番茄植株根结数分别比空载体对照减少了59.6%,比清水对照降低了59.5%。结果表明,MiAsg基因沉默对根结线虫病害具有很好的防控效果,也说明MiAsg基因可能参与线虫的致病性。Root-knot nematode （Meloidogyne spp.） is a worldwide disease. In preliminary stuales, we precnct me number of functional genes of nematode in its genome using bioinformatics methods. In this study, we designed special primers based on the predicted mitochondrial ATP synthase g subunit gene （A sg） sequence and cloned the A sg gene in M. incognita （MiA sg） which was transferred into tomato plantlets using virus-induced gene silence （VIGS） technique after analyzing the sequence characters to investigate the relationship between MiA sg gene and the disease caused by root-knot nematode. The results showed that the similarity between the cloned MiAsg gene and the predicted Asg was as high as 100%. Sixty days after inoculation with M. incognita, the amounts of root knot on MiAsg gene silence-treated tomato plantlets decreased by 59.6% compared to that of the empty vector control, by 59.5% compared to that of the water control. The study revealed the MiAsg gene silence had a good effect on prevention and control of root-knot nematode disease, also showed the MiAsg gene may be involved in the pathogenesis of nematode.

关 键 词：根结线虫 RNA干扰 线粒体ATP合成酶g亚基 病毒诱导的基因沉默 

分 类 号：S432.45[农业科学—植物病理学]