SPR免疫传感技术检测水中阿特拉津除草剂  

DETECTION OF ATRAZINE HERBICIDE IN WATER USING SURFACE PLASMON RESONANCE IMMUNOSENSOR

作　　者：柳明[1] 祁军[2] 刘磊 张园[1] 赵琢[1] 王华[1] 刘伟[1] 孙俐[1] 彭媛[4] 白家磊[4] 高志贤[4] 

机构地区：[1]天津出入境检验检疫局工业产品安全技术中心,天津300201 [2]天津国际旅行卫生保健中心 [3]天津市农业质量标准与检测技术研究所 [4]军事医学科学院卫生学环境医学研究所 天津市环境与食品安全风险监控重点实验室

出　　处：《解放军预防医学杂志》2013年第4期299-301,共3页Journal of Preventive Medicine of Chinese People's Liberation Army

基　　金：国家自然科学基金青年科学基金项目(No.21207161;No.21107142)

摘　　要：目的利用表面等离子体共振（sPR）生物传感技术，对水样中的三嗪类农药阿特拉津进行检测．为研发相应快检技术与设备提供技术基础。方法以抗原或抗体作为传感器敏感识别元件，利用间接竞争法结合SPR传感技术对阿特拉津进行检测。结果方法最低检出限2．34ng／ml（S／N=3），IC50=32．36ng／ml，检测范围5．75～181．97ng／ml，检测时间〈30min，回收率98．4％～104．2％，相对标淮偏差1．9％。结论所建立方法对于检测水样中阿特拉津具灵敏性、准确性和精密性。Objective In the purpose of providing fast detection technology and equipment, a detection method for atrazine in water samples using surface plasmon resonance （SPR） biosensor was established. Methods Antigen and antibody was used as sensitive recognition sensor element, and indirect competitive assay combined with SPR sensing technology was employed to detect atrazine. Results The minimum detection limit of the estab- lished method was 2.34 ng/ml （ S/N =3 ）. The half maximal inhibitory concentration（ IC50 ） was 32.36 ng/ml, de- tection range 5.75 - 181.97 ng/ml, and detection time less than 30 min. The recovery rate ranged from 98.4% to 104.2% and relative standard deviation was 1.9%. Conclusion The established detection method for atrazine in water is sensitive, accurate, and precise.

关 键 词：表面等离子体共振 阿特拉津除草剂 抗原抗体 生物传感检测 

分 类 号：R392[医药卫生—免疫学]