毛细管电泳法同时测定尿液中百草枯和肌酐  

Simultaneous detection of paraquat and creatinine in urine by capillary electrophoresis

作　　者：吉艳[1] 吴惠毅[1] 赵绍林[1] 杨晋[1] 陈晓兵[2] 李小民[2] 

机构地区：[1]连云港市第一人民医院中心实验室,222002 [2]连云港市第一人民医院急诊医学科,222002

出　　处：《中华检验医学杂志》2013年第9期791-795,共5页Chinese Journal of Laboratory Medicine

基　　金：连云港市第一人民医院青年英才豪森基金项目（QN110301）;连云港市＂科教兴卫工程＂青年科技项目（Q1203）

摘　　要：目的 建立一种毛细管电泳法同时检测尿液百草枯（PQ）和肌酐（Cr）含量的方法.方法 实验方法学研究.收集201 1年1月至2012年6月连云港市第一人民医院就诊急性PQ中毒患者8例,男2例,女6例.在毛细管区带电泳模式下,以25 mmol/L pH 1.97甘氨酸-HCl（含40 mmol/LNaCl）为电泳缓冲液,在非涂层石英毛细管（47 cm×75 μm内径）中进行电泳.尿液标本离心后用H2O稀释10倍后直接压力进样4s,20 kV电压分离后通过二级阵列管检测器检测,检测波长200 nm.对该法进行系统的方法学评价（线性范围及检测限,重复性实验,回收实验,干扰实验）,测定中毒患者尿液中PQ和Cr含量.结果 PQ和Cr均在5 min内基线分离.PQ和Cr分别在2～ 1000μmol/L、10 ～ 5000 μmol/L范围内呈良好的线性,相关系数分别为0.9997和0.9999 （P＜0.01）,检出限分别为1.0、5.0 μmol/L.尿中PQ和Cr峰面积的平均日内（n=10）CV为2.84％、1.72％,平均日间（n=10） CV为3.62％、3.06％.PQ和Cr的平均回收率分别为88.6％、90.2％.敌草快（DQ）对PQ测定无干扰.PQ中毒患者（n=8）尿液标本的PQ/Cr（μmol/mmol）比值在8.9 ～ 2215之间.结论 成功建立了毛细管电泳法快速测定尿液中PQ和Cr的方法.该法可同时检测PQ中毒患者尿液中的PQ和Cr含量,标本用量少,且离心后稀释直接进样,操作简便快速,结果准确,易于自动化,可用于PQ中毒患者尿液标本的快速测定.Objective To establish a method for detecting the concentration of paraquat （PQ） and creatinine（Cr） in urine simultaneously by capillary electrophoresis. Methods Experimental methodological study. 8 acute PQ poisoned patients who were treated in the First People＇s Hospital of Lianyungang from January 2011 to June 2012 were collected. 2 were male, and 6 were female. The separation were carried out using a 25 mmoL/L pill. 97 glycine-HC1 buffer（ containing 40 mmol/L NaC1） in a fused-silliea capillary tube of 47 cm×75μ m I.D. by capillary zone electrophoresis. Urine had been injected by pressure for 4 s after samples were centrifuged and diluted for 10 times with H20. The detection were monitored by a diode-array detector at 200 nm while samples were separated at a voltage of 20 kV. A systemic methodological evaluation of this method was carried out （The linear range, detection limit, repeatability test, recovery test and interference test）. And the method was used to detect the concentration of PQ and Cr in PQ poisoned patients＇ urine. Results The peaks of PQ and Cr appeared within 5 rain. The linear ranges of PQ and Cr were 2-1000, 10-5000 μmol/L, respectively, with the correlation coefficients of 0. 9997 and 0. 9999 （P 〈 0.01 ）. The detection limits were 1.0 μmol/L for PQ and 5.0 txmol/L for Cr. The mean within-day（ n = 10） CVs of peak area for PQ and Cr were 2.84% and 1.72%, while the mean inter-day（n = 10） CVs of peak area were 3.62% and 3.06%. The average recovery rate of PQ and Cr were 88.6% and 90, 2% respectively. Diquat（DQ） didn＇t interfere with the assay. The range of PQ/Cr（txmol/mmol） for 8 cases was 8.9 - 2215. Conclusions A method was established successfully for the rapid determination of PQ and Cr in urine by capillary electrophoresis. The CE method devised here for direct measurement of urinary PQ and Cr from PQ poisoned patients is simple, fast, automatic and with good repeatability. It is an ideal method for rapid detection of urinary PQ in PQ poisone

关 键 词：电泳 毛细管 尿分析 百草枯 肌酸酐 中毒 

分 类 号：R284.1[医药卫生—中药学]