乙肝病毒DNA、前S1抗原及核心抗体IgM的临床应用  

Clinical application of hepatitis B virus DNA,PreS1 antigen and core antibody IgM

作　　者：彭俊华[1] 常亚丽[1] 柴玉香[2] 曹晶晶[1] 

机构地区：[1]兰州军区兰州总医院检验科,甘肃兰州730050 [2]甘肃省黄河医院检验科

出　　处：《西北国防医学杂志》2013年第5期407-409,共3页Medical Journal of National Defending Forces in Northwest China

摘　　要：目的:探讨乙肝病毒前S1抗原(PreS1-Ag)、乙肝病毒核心抗体IgM(HBcAb-IgM)与HBV-DNA的相关性及其应用。方法:用酶免法检测PreS1-Ag、HBcAb-IgM,用实时定量荧光PCR扩增HBV-DNA。结果:PreS1-Ag、HBcAb-IgM和HBV-DNA的阳性率在HBsAg/HBeAg/HbcAb(+)组分别为78.92%、28.25%和88.79%,明显高于HBsAg/HBeAb/HbcAb(+)组分别为56.39%、6.05%和35.81%;PreS1-Ag、HBcAb-IgM和HBV-DNA阳性率在HBeAg(+)组分别为76.56%、26.37%和87.91%,明显高于HBeAg(-)组,分别为55.27%、5.85%和40.16%。PreS1-Ag判断HBV复制的敏感率明显高于HBV-DNA,而HBeAg和HBcAb-IgM明显低于HBV-DNA。结论:PreS1-Ag、HBcAb-IgM和HBV-DNA与HBeAg有很好相关性,与HBV五项血清标志物联用有良好的应用前景。Objective： To investigate the correlation and its application of hepatitis B virus DNA （HBV - DNA）, PreS1 antigen （PreS1 -Ag） and core antibody IgM （HBcAb -IgM）. Methods： The PreS1 -Ag and HB- cAb - IgM were detected by enzyme immunoassay, and the HBV - DNA was amplified by real - time fluorescence quantitative PCR. Results：The positive rates of PreS1 - Ag, HBcAb - IgM and HBV - DNA were 78.92%, 28.25% and 88.79% respectively in HBsAg/HBeAg/HbcAb （ ＋ ） group, and 56.39%, 6.05% and 35.81% respectively in HBsAg/HBeAb/HbcAb （ ＋ ） group （P 〈0.01 ）. The positive rates of PreS1 - Ag, HBcAb - IgM and HBV - DNA were 76.56% , 26.37% and 87.91% respectively in HBeAg （ ＋ ） group, and 55.27% , 5.85% and 40.16% respectively in HBeAg （ - ） group （P 〈 0.01 ）. For evaluating HBV replication, the sensitivity of PreS1 - Ag was significantly higher than that of HBV - DNA （ P 〈0.01 ）, and those of HBeAg and HBcAb - IgM were significantly lower than that of HBV - DNA （P 〈 0.01 ）. Conclusion： PreS1 - Ag, HBcAb - IgM and HBV - DNA are significantly correlated with HBeAg, and combination detection of them with five serum HBV markers will be prospective for clinical application.

关 键 词：乙肝病毒 前S1抗原 HBV-DNA HBcAb-IgM 实验技术 

分 类 号：R512.62[医药卫生—内科学]