达氏鲟视网膜早期发育及其相关机能  

Retina development and correlated function during Dabry's sturgeon(Acipenser dabryanus) ontogeny

作　　者：史玲玲[1,2] 危起伟[1,2,3] 柴毅[2,4] 杜浩[1,2,3] 李罗新[2,3] 谢晓[1,2] 

机构地区：[1]华中农业大学水产学院,湖北武汉430070 [2]农业部淡水生物多样性保护重点实验室,中国水产科学研究院长江水产研究所,湖北武汉430223 [3]中国水产科学研究院淡水渔业研究中心,江苏无锡214081 [4]长江大学动物科学学院,湖北荆州434025

出　　处：《中国水产科学》2013年第5期958-967,共10页Journal of Fishery Sciences of China

基　　金：农业部公益性行业科研专项经费资助项目(200903048-02)

摘　　要：本研究采用石蜡连续切片技术, 对出膜后0-64 d 的达氏鲟（Acipenser dabryanus）视网膜结构、视觉特性及相关机能进行研究。结果显示, 达氏鲟初孵仔鱼的视网膜没有分化, 视单锥细胞在36 h 出现, 视杆细胞在6 d 时出现, 7 d 时视网膜各层分化完毕。最小分辨角由8 d 的13.26′下降到64 d 的3.37′, 仔鱼发育到13 d 时表现出明显的视网膜运动反应。随着仔鱼发育, 视单锥细胞及神经节细胞密度不断降低, 视杆细胞密度不断增加, 外核层细胞核与视锥细胞及神经节细胞数量的比值均不断增大。结果表明6-12 d 是达氏鲟视网膜结构快速发育和视觉特性发生明显变化的时期, 这种变化与其生态变迁、趋光性及摄食方式的变化相适应。本研究旨在探明达氏鲟视网膜的发育特性及其相关机能特点, 为开展规模化繁育与种群资源的保护提供理论依据。We studied the retina structure, visual characteristics, and correlated function of Dabry＇s sturgeon （Acipenser dabryanus） ranging from newly hatched larvae to 64-day-old juveniles using paraffin serial sections. The main results are as follows： Larvae exhibited undifferentiated retina at hatching. Single cones appeared after 36 hours and rods appeared after 6 days. After 7 days, the visual system had completely differentiated. The apparent retinomotor responses first occurred on day 13. However, the minimum separable angle （α） decreased from 13.26′ on day 8 to 3.37′ on day 64. During the developmental period, the density of single cones and ganglion cells decreased while that of the rods correspondingly increased. The ratio of nuclei of the outer nuclear layer to ganglion cells per 100 μm increased, as did the ratio of nuclei of the outer nuclear layer to single cones. Our investigation revealed that the retina structure and visual characteristics changed rapidly from day 6 to 12 to adapt to ecological shifts and changes in photo taxis and feeding behavior.

关 键 词：达氏鲟 视网膜 早期发育 相关机能 

分 类 号：Q954[生物学—动物学]