无创性延迟肢体缺血预适应对大鼠缺血再灌注损伤心肌持续保护效应  

Durative protection of noninvasive delayed limb ischemic preconditioning against myocardial ischemia-reperfusion injury in rats

作　　者：高建波[1,2] 张颖[2] 娄建石[2] 

机构地区：[1]天津市药品检验所 [2]天津医科大学药理学教研室,天津300070

出　　处：《中国病理生理杂志》2013年第9期1691-1695,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81072631);天津市自然科学基金资助项目(No.09JCZDJC21100)

摘　　要：目的:探讨连续不同天数的无创性肢体缺血预适应对大鼠缺血再灌注损伤心肌的延迟保护持续时间的差别。方法:雄性Wistar大鼠随机分为:(1)对照组:包括假手术组、缺血再灌注组、心肌缺血预适应组和股动脉缺血预适应组;(2)无创性延迟肢体缺血预适应组:包括1 d远端肢体缺血预适应间隔1 d组、3 d组和5 d组,3 d远端肢体缺血预适应间隔1 d组、3 d组和5 d组,7 d远端肢体缺血预适应间隔1 d组、3 d组和5 d组。每组分别在间隔末期进行缺血再灌注损伤,监测缺血再灌注期间左心室功能、心律失常情况和S-T段升高幅度,并检测再灌注结束时心型脂肪酸结合蛋白(heart fatty acid binding protein,H-FABP)、糖原磷酸化酶BB(glycogen phosphorylase BB,GPBB)及心肌梗死面积。结果:与缺血再灌注组相比,心肌缺血预适应组、股动脉缺血预适应组、3 d和7 d远端肢体缺血预适应间隔1 d组明显改善左心室功能,减少心律失常,降低S-T段抬高幅度,降低H-FABP和GPBB活性,减少心肌梗死面积。结论:远端肢体缺血预适应3 d与7 d可获得相似的心肌持续保护效应。AIM：To test the difference of the duration of delayed protection among different continuous days of noninvasive delayed limb ischemic preconditioning （NDLIP） for cardioprotection against ischemia-reperfusion （I/R） injury in rats. METHODS：Male Wistar rats were randomized into the following groups： （1） control groups, including sham operation, I/R, myocardial ischemic preconditioning （MIPC） and femoral artery ischemic preconditioning （FAIP） groups; （2） NDLIP groups, including 1-day NDLIP plus 1-day interval （NDLIP1d＋1d）, 1-day NDLIP plus 3-day interval （NDLIP1d＋3d）, 1-day NDLIP plus 5-day interval （NDLIP1d＋5d）, 3-day NDLIP plus 1-day interval （NDLIP3d＋1d）, 3-day NDLIP plus 3-day interval （NDLIP3d＋3d）, 3-day NDLIP plus 5-day interval （NDLIP3d＋5d）, 7-day NDLIP plus 1-day interval （NDLIP7d＋1d）, 7-day NDLIP plus 3-day interval （NDLIP7d＋3d） and 7-day NDLIP plus 5-day interval （NDLIP7d＋5d） groups. Myocardial I/R injury was performed at the end of the intervals. The left ventricular function, ventricular arrhythmia incidence and ST-segment were measured during I/R. Myocardial infarct size, heart fatty acid-bin-ding protein （H-FABP） and glycogen phosphorylase BB （GPBB） were determined at the end of the experiment. RESULTS：Compared with I/R group, MIPC, FAIP, NDLIP3d＋1d and NDLIP7d＋1d groups showed attenuated ventricular arrhythmia, improved left ventricular function, lowered ST-segment elevation, reduced myocardial infarct size, and decreased H-FABP and GPBB activity. CONCLUSION：NDLIP for 3 and 7 days can provide the similar duration of cardioprotection in rats.

关 键 词：肢体缺血预适应 心肌缺血再灌注 心肌保护 

分 类 号：R96[医药卫生—药理学]