胰岛素对严重烧伤早期大鼠心肌氧化应激的影响  

Effect of insulin on early myocardial oxidative stress in severely burnt rats

作　　者：王伟[1] 蒋红梅[2] 李伟人[1] 孔豫苏[1] 鲁加祥[1] 李嘉琥[1] 喻翔[1] 

机构地区：[1]贵阳医学院附属医院烧伤整形科,贵州贵阳550004 [2]贵阳医学院医学检验系,贵州贵阳550004

出　　处：《中国病理生理杂志》2013年第9期1709-1711,1724,共4页Chinese Journal of Pathophysiology

基　　金：贵州省科技教育人才省长专项基金资助项目[No.黔省专合字(2008)92];贵州省卫生厅科学技术基金资助项目(No.gzwkj2011-1-022)

摘　　要：目的:探讨胰岛素对严重烧伤早期大鼠心肌氧化应激的影响。方法:24只SPF级SD大鼠,随机分为3组,分别是对照组(假烫伤组)、烫伤组和烫伤+胰岛素组。后2组大鼠背部造成30%烧伤总面积(TBSA)的Ⅲ度烫伤,伤后立即腹腔注射生理盐水(40 mL/kg)。烫伤+胰岛素组大鼠复苏补液后皮下注射胰岛素(1 U/kg),烫伤组同法注射等量生理盐水。于伤后24 h采集腹主动脉血和心脏组织标本。采用分光光度法测定血糖(BG)、乳酸脱氢酶(LDH)和肌酸激酶(CK)活性及心脏组织中氧化、抗氧化指标,包括丙二醛(MDA)含量、黄嘌呤氧化酶(XO)、髓过氧化物酶(MPO)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GPx)活性。结果:(1)烫伤组BG含量与对照组比较显著升高(P<0.05),烫伤+胰岛素组BG水平较烫伤组显著降低(P<0.05),与对照组比较显著升高(P<0.05)。(2)烫伤组LDH和CK活性与对照组比较显著升高(P<0.05),烫伤+胰岛素组LDH和CK活性与烫伤组比较显著降低(P<0.05)。(3)烫伤组心肌MDA含量、XO与MPO活性与对照组比较显著升高(P<0.05),SOD、CAT和GPx活性与对照组比较显著降低(P<0.05);烫伤+胰岛素组心肌MDA含量、XO与MPO活性较烫伤组显著降低(P<0.05),SOD、CAT和GPx活性较烫伤组显著升高(P<0.05)。结论:胰岛素干预减轻烧伤后早期大鼠心肌的氧化应激,使升高的心肌酶活性下降,呈现心肌保护效应。AIM：To investigate the effect of insulin on early myocardial oxidative stress in severely burnt rats. METHODS：Twenty-four Sprague-Dawley rats were randomly divided into three groups （8 rats in each group）： control group （sham scald group）, scald injury group and scald injury ＋ insulin group. The rats in the latter two groups were subject to third-degree burn with 30% total burn surface area （TBSA） on the back, and then received intraperitoneal injection of normal saline （40 mL/kg） immediately. The rats in scald injury ＋ insulin group were subcutaneously injected with insulin （1 U/kg）, while those in scald injury group received subcutaneous injection of the same volume of normal saline. All rats were sacrificed 24 h after scald, and blood samples from abdominal aorta and myocardial tissues were taken. Blood glucose （BG） content, blood lactate dehydrogenase （LDH） and creatine kinase （CK） activity, and myocardial oxidative and antioxidative indexes, including malondialdehyde （MDA）, xanthine oxidase （XO）, myeloperoxidase （MPO）, superoxide dismutase （SOD）, catalase （CAT） and glutathion peroxidase （GPx）, were detected by spectrophotometry. RESULTS：（1） Compared with control group, BG levels in scald injury group and scald injury ＋ insulin group were significantly elevated （P〈0.05）. But BG in scald injury ＋ insulin group was significantly lower than that in scald injury group （P〈0.05）. （2） Compared with control group, the activity of LDH and CK in scald injury group was significantly increased （P〈0.05）, while that in scald injury ＋ insulin group was significantly lower than that in scald injury group （P〈0.05）. （3） Compared with control group, the MDA content and the XOD and MPO activity in scald injury group were significantly increased （P〈0.05）, while the activity of SO, CAT and GPx was significantly decreased （P〈0.05）. Compared with scald injury group, the MDA content and the XO and MPO activity in

关 键 词：烧伤 胰岛素 心肌 氧化性应激 

分 类 号：R322.11[医药卫生—人体解剖和组织胚胎学]