EGCG对百草枯致小鼠急性肺损伤的保护作用  

Protective Effect of EGCG Against Paraquat Induced Acute Lung Injury in Mice.

作　　者：江民礼[1] 吕利雄[1] 

机构地区：[1]上海交通大学医学院附属仁济医院急诊与危重病学科,200127

出　　处：《医学研究杂志》2013年第9期47-51,共5页Journal of Medical Research

基　　金：上海市公共卫生优秀学科带头人培养计划基金资助项目(08GWD16)

摘　　要：目的研究表没食子儿茶素没食子酸酯(EGCG)在百草枯(paraquat,PQ)致小鼠急性肺损伤中的保护作用。方法72只C57BL小鼠随机分为空白对照(NS)组,百草枯对照(PQ)组,EGCG对照组,EGCG处理组。空白对照组给予生理盐水60mg/kg腹腔注射,百草枯组给予PQ60mg/kg腹腔注射。EGCG对照组和EGCG处理组给予EGCG 4.0mg/kg腹腔注射。给药后观察各组小鼠肺组织病理改变,酶联免疫吸附(ELISA)法测定血清中肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)及高迁移率蛋白(high-mobility group box 1,HMGB1)的含量,real-time PCR法测定肺组织TNF-α,HMGB1 mRNA表达水平。并观察HMGB1在肺组织中表达的改变。结果百草枯对照组小鼠染毒后6h血清及肺组织TNF-α、HMGB1水平即明显高于NS对照组(P<0.05)。经EGCG处理组小鼠百草枯中毒第3天血清HMGB1和TNF-α水平低于PQ对照组(P<0.05或P<0.01)。与百草枯对照组小鼠相比,EGCG处理组小鼠百草枯染毒后肺组织炎性改变明显减轻,肺组织HMGB1表达也明显减弱。结论EGCG可明显减轻百草枯中毒导致的急性肺损伤。Objective To study the protective effect of epigallocatechin-3-gallate （EGCG） against PQ induced acute lung injury.Methods Seventy-two C57BL mice were randomly divided into four groups：normal saline group,control group of paraquat （60mg/kg）,EGCG group,and paraquat plus EGCG group.The drug was intraperitoneally injected,and EGCG （4.0mg/kg） was intraperitoneally injected at 2,12 and 24h post paraquat intoxication.They were sacrificed to measure the content of serum tumor necrosis factor-α （TNF-α）,and high-mobility group box 1 （HMGB1）,and determine the lung tissue HMGB1 mRNA and TNF-α mRNA expression level with R-T PCR method.The pathologic changing of lung tissue was observed and lung HMGB1 expression was assessed by immunohistochemistry.Results The serum and the lung level of TNF-α,HMGB1 in the control group of paraquat were obviously increased in the sixth hour.Compared with control group of paraquat,mice treated with EGCG had significantly lower level of TNF-α and HMGBI.The infiltration of inflammatory cells and structural damage of lung tissue were reduced in the treatment group of EGCG.Lung immunohistochemical staining of HMGB1 showed that lung HMGB1 expression was also significantly reduced.Conclusion Our results suggest that treatment with EGCG could significantly reduce paraquat induced acute lung injury.

关 键 词：EGCG 百草枯 高迁移率蛋白 急性肺损伤 

分 类 号：R338[医药卫生—人体生理学]