广东铁皮石斛GAP基地的石斛鲜品茎和叶中多糖的含量比较研究  

Comparative Study on Polysaccharides Content in Stem and Leaf of Iron Stone Dendrobium Fresh Product from different producing place.

作　　者：鲁芹飞[1] 王培培[1] 陈建南[1,2] 黄松[1,2] 

机构地区：[1]广州中医药大学,510006 [2]广州中医药大学中医药数理工程研究院

出　　处：《医学研究杂志》2013年第9期55-58,共4页Journal of Medical Research

基　　金：广东省科技计划项目(2011A030100011);广东省科技计划项目(2008A0301009);广东省科技教育部产学研结合项目(2010B090400474)

摘　　要：目的通过对不同产地铁皮石斛引种到广东饶平铁皮石斛GAP种植基地的铁皮石斛药材鲜品茎和叶中的多糖含量比较研究,探讨环境因素对石斛多糖的影响。方法以多糖含量为指标,应用苯酚-浓硫酸比色法测定不同产地的铁皮石斛鲜品茎和叶中的多糖含量。结果不同原产地的铁皮石斛茎和叶中的多糖含量均有一定差异,其中鲜品的茎以云南-5(T8)样品的多糖含量最高,达到了5.59%,广东河源的样品的多糖含量最低,仅为1.22%;鲜品的叶以云南-4(T7)样品的多糖含量最高,达到了1.90%,福建武夷山的样品的多糖含量最低,为0.95%;多数品种的鲜品茎中的多糖含量高于叶中的多糖含量。结论原产地的生长环境对铁皮石斛多糖的积累可能有一定影响。Objective To establish a method for the determination of polysaccharide content in the stems and leaves of Iron stone dendrobium Fresh Product from different producing place,and to explore the influence of environment on the polysaccharide constituents in the stems and leaves.Methods Phenol-sulfuric acid method was applied for the determination of polysaccharide in the stems and leaves of Iron stone dendrobium Fresh Product.Results Polysaccharides content in stem and leaf of Iron stone dendrobium Fresh Product from different producing place was different.Polysaccharides content in stems of Yunnan-5 （T8） sample was the highest （5.59%）,while it was the lowest in GuangdongHeyuan districts sample （1.22%）.Polysaccharides content in leaves of Yunnan-4 （T7） sample was the highest（1.90%）,while it was the lowest in FujianWuyishan districts sample（0.95%）.It＇s common that polysaccharides content in fresh stems was higher than it in leaves.Conclusion Different growing environment of Iron stone dendrobium could probably influence the Polysaccharides content in it.

关 键 词：铁皮石斛 鲜品 多糖 含量测定 苯酚-浓硫酸法 

分 类 号：R282.71[医药卫生—中药学]