初发系统性红斑狼疮患者CD4^+CD25^+CD127^(low/-)T细胞可能具有效应T细胞特点的研究  

CD4^+CD25^+CD127^(low/-)T Regulatory Cells May Have the Characteristics of Effector T cells in Patients with New-onset Systemic Lupus Erythematosus

作　　者：赵书山[1] 王健[1] 厉伟民[2] 

机构地区：[1]温州医学院附属东阳医院风湿免疫科,322100 [2]温州医学院附属东阳医院内科,322100

出　　处：《医学研究杂志》2013年第9期109-113,共5页Journal of Medical Research

摘　　要：目的检测外周血CD4+CD25+CD127low/-T细胞和CD4+CD25highCD127low/-T细胞,探讨调节性T细胞在系统性红斑狼疮发病中的作用。方法采用三色直接荧光素标记法和多参数流式细胞仪检测32例SLE患者及24名正常对照者外周血CD4+CD25+CD127low/-T细胞、CD4+CD25highCD127low/-T细胞的比例,同时检测CD4+CD25+CD127low/-T细胞的FOXP3表达水平及CD4+CD25+CD127low/-T细胞培养上清液IL-17和TGFE-β1的表达水平。结果 SLE患者外周血CD4+CD25high CD127low/-T细胞与正常对照组比较明显减少,差异有统计学意义(P<0.001);然而,CD4+CD25+CD127low/-T细胞与正常对照组比较无显著性差异(P>0.05)。CD4+CD25highCD127low/-T细胞与SLEDAI评分呈负相关(P<0.001)。CD4+CD25+CD127low/-T细胞与SLEDAI评分无相关性(P=1.000)。与正常对照相比,初发SLE患者CD4+CD25+CD127low/-T细胞培养基产生较多的IL-17(P<0.001),而TGF-β1的产生减少(P<0.001)。与正常对照相比,初发SLE患者CD4+CD25+CD127low/-T细胞的FOXP3表达减少(P<0.001)。结论初发系统性红斑狼疮患者CD4+CD25+CD127low/-T细胞可能具有效应性T细胞特点。Objective To detect Peripheral blood CD4 ＋ CD25 ＋ CD127low/-T cells and CD4 ＋ CD25highCD127low/-T cells in patients with systemic lupus erythematosus,in order to reveal the role of Treg cells in the pathogenesis of SLE.Methods Peripheral blood CD4 ＋CD25 ＋ CD127low/-T cells,and CD4 ＋ CD25highCD127low/-T cells in 32 SLE patients and 24 healthy volunteers were analyzed by 3-color flow cytometry.The percentage of FOXP3 in CD4 ＋ CD25 ＋ CD127lOW/-Tregs and the level of IL-17/TGFβ1 in CD4 ＋ CD25 ＋ CD127low/-Tregs cultures were also measured.Results Our results showed that peripheral blood CD4 ＋ CD25highCD127low/-Tregs in SLE patients were signifiantly lower than those of the control group（P 〈 0.001）.However,the levels of CD4 ＋ CD25 ＋ CD127low/-Tregs in patients with SLE didn＇t show a significant change compared with control group （P 〉 0.05）.The CD4 ＋ CD25highCD127low/-Tregs were negatively correlated with systemic lupus erythematosus disease activity index （SLEDAI） （P 〈 0.001）.But,there was no significant correlation between CD4 ＋ CD25 ＋ CD127low/-Tregs and SLEDAI（P =1.000）.IL-17 levels were significantly increased in CD4 ＋ CD25 ＋ CD127low/-Tregs cultures in SLE patients compared with normal controls（P 〈 0.001）,whereas the level of TGFβ1 was low in CD4 ＋ CD25 ＋ CD127low/-Tregs cultures compared with normal controls（P 〈0.001）.The percentage of FOXP3 in CD4 ＋ CD25 ＋ CD127low/-Tregs were lower in in patients with new-onset SLE compared with normal controls （P 〈 0.001）.Conclusion As a result,CD4 ＋ CD25 ＋ CD127low/-T regulatory cells may have the characteristics of effector T cells in patients with new-onset systemic lupus erythematosus.

关 键 词：调节性T细胞 系统性红斑狼疮 流式细胞术 

分 类 号：R392.11[医药卫生—免疫学]