马齿苋总黄酮对缺血缺氧刺激下血管平滑肌细胞蛋白激酶C活性的影响  

Effects of portulaca total flavone on the influence of vascular smooth muscle cell calcium overload and protein kinase C induced by hypoxia

作　　者：卢新华[1] 王桂霞[1] 何军山[2] 

机构地区：[1]湘南学院基础医学部,湖南郴州423000 [2]湘南学院预防检验系,湖南郴州423000

出　　处：《中国临床药理学杂志》2013年第9期685-688,共4页The Chinese Journal of Clinical Pharmacology

基　　金：湖南省自然科学基金资助项目(06JJ50064);湖南省教育厅十二.五重点建设学科基金资助项目;湖南省心脑血管天然药物研究重点实验室基金项目资助

摘　　要：目的观测马齿苋总黄酮(PTF)对缺血缺氧刺激下血管平滑肌细胞钙超载及蛋白激酶C(PKC)的影响。方法用Fura-2/AM作Ca2+指示剂,检测PTF对正常培养及缺血缺氧作用下家兔主动脉血管平滑肌细胞Ca2+浓度及PKC的活性的改变。结果 PTF对正常培养家兔主动脉血管平滑肌细胞[Ca2+]i浓度无明显影响;PTF(8,16,32,64 mg·L-1)剂量依赖性抑制缺血缺氧缺氧致血管平滑肌细胞[Ca2+]i升高;PTF对正常培养家兔主动脉血管平滑肌细胞胞浆、胞膜PKC活性均升高;PTF处理后胞浆PKC活性下降,胞膜PKC活性上升。结论PTF可能抑制缺氧致血管平滑肌细胞钙超载,调节缺氧条件下血管平滑肌细胞PKC活性。Objective To explore the effects of portulaca total flavones （PTF） on the influence of vascular smooth muscle cell calcium overload and protein kinase C （PKC） induced by hypoxia. Methods The effect of PTF on intracellular free calcium concentration and the activity of PKC were determined in normal cultured and during hypoxial rabbit vascular smooth muscle cells（VSMC）. The fluorescent Ca^2＋ -indicater fura-2/ AM was used. The effects of PTF were compared with that of amlodipine. The ischemia and hypoxia model was made, then the activity of PKC was measured by γ scintillation counting instrument. Results It have no ob-vious influence that PTF on normal training rabbit aortic VSMC [ Ca^2＋ ] I concentration. PTF （8,24,72 mg · L^-1） inhibited the elevation of [ Ca^2＋] i induced by hypoxial depolarization in a concentration depend-ent manner. It was found that the activity of PKC treated with PTF in VSMC cytoplasm and cell membrane in normal condition increased . The activity of PKC in cytoplasm in ischemia and hypoxia Situation increased, but the activity of PKC in cell membrane decreased. When VSMC was treated with PTF, the activity of PKC in cytoplasm＇decreased and that of cell membrane increased. Conclusion [ Ca^2＋ ] i of VSMC by blocking calcium The PTF might inhibited the channel, could regulate the PKC activities in hypoxial.

关 键 词：马齿苋总黄酮 血管平滑肌细胞 钙超载 缺血缺氧 蛋白激酶C 

分 类 号：R285.5[医药卫生—中药学] R965[医药卫生—中医学]