醋柳黄酮及其单体抑制大鼠心肌肥大的实验研究  

Experimental study on the inhibition of rat myocardial hypertrophy with total flavones of hippophae rhamnoides l(tfh) and its monomer

作　　者：宋鑫[1] 余琴[2] 刘应才[2] 

机构地区：[1]四川省合江县人民医院心内科,四川合江646200 [2]四川省泸州医学院附属医院心内科,四川泸州646000

出　　处：《中国现代医学杂志》2013年第22期20-25,共6页China Journal of Modern Medicine

摘　　要：目的研究醋柳黄酮(TFH)及其单体槲皮素(Quer)和异鼠李素(Isor)抑制心肌细胞肥大的机制是否与钙调神经磷酸酶(CaN)信号通路相关。方法在培养原代大鼠心肌细胞的基础上,实验采用血管紧张素Ⅱ(AngⅡ)诱导新生大鼠心肌细胞肥大为模型,分别加入TFH、Quer、Isor和缬沙坦(Valsartan)进行预防性治疗。鉴定心肌细胞,检测心肌细胞表面积及总蛋白含量,测定心肌细胞肌浆网钙泵(Sarcoplasmic reticulum Ca2+-ATPases,SERCA)活力、CaN活性及CaN mRNA表达。结果实验培养的心肌细胞抗α-actin免疫细胞化学染色均为阳性;AngⅡ组细胞表面积增大,总蛋白含量增加,与对照组相比差异有显著性(P<0.01);TFH组、Quer组、Isor组和Valsartan组均能显著降低心肌细胞肥大(P<0.01或P<0.05)、提高SERCA活力(P<0.05)、降低CaN活性(P<0.05)及CaN mRNA(P<0.01)表达,而这四组之间差异无显著性(P>0.05)。结论醋柳黄酮及其单体(槲皮素和异鼠李素)与缬沙坦一样,对AngⅡ诱导心肌细胞肥大有明显的抑制作用,其作用机制可能与心肌细胞肌浆网钙泵活性及钙调神经磷酸酶信号通路有关。[Objective] To study whether the inhibition mechanism for cardiomyocyte hypertrophy of the total flavones of Hippophae rhamnoides （TFH） and its monomer Quercetin （Quer） and isorhamnetin （Isor） are associated with the signal passage of calcineurin （CAN）. [Method] Based on the cultivation of primary rat cardiomyocytes, the an- giotensin Ⅱ （Ang Ⅱ ） was used to induce neonatal rat cardiomyocyte hypertrophy as a model in the experiment, and TFH, Quer, Isor and valsartan were added for preventive treatment. The cardiomyoeytes were identified, the surface area and total protein content of cardiomyocyte were detected, and the vitality of Sarcoplasmic reticulum Ca2＋-ATPases （SERCA）, the CaN activity as well as the expression of CaN mRNA were determined. [Results] The anti-α-actin im- munocytochemical staining for the cultured cardiomyocytes was positive in the experiment; the surface area of Ang group＇s cell enlarged, the total protein content increased; And compared with the control group, the difference was of statistical significance （P 〈0.01）. The cardiomyocyte hypertrophy in the TFH, Quer, Isor and Valsartan groups could significantly reduced （P 〈0.01 or P 〈0.05）, the SERCA vitality improved （P 〈0.05）, and the CaN activity（P 〈0.05） and CaN mRNA （P 〈0.01） expression were lowered. The differences among the four groups were of no statistical signifi- cance （P 〉0.05）. [ Conclusion] Like valsartan, TFH and its monomer （quercetin and isorhamnetin） has a significant in- hibition effect on the Ang Ⅱ-induced cardiomyocyte hypertrophy, and its mechanism may be associated with the ac-tivity of Sarcoplasmic reticulum Ca2＋-ATPases （SERCA） and the signal passage of calcineurin.

关 键 词：醋柳黄酮 血管紧张素Ⅱ 心肌肥大 钙调神经磷酸酶 

分 类 号：R542.2[医药卫生—心血管疾病] R965[医药卫生—内科学]