香港巨牡蛎♀×近江牡蛎♂受精及早期胚胎发育过程的荧光显微观察  

FERTILIZATION AND EARLY EMBRYOGENESIS OF CRASSOSTREA HONGKONGENSIS♀×C.ARIAKENSIS♂ WITH FLUORESCENT MICROSCOPE

作　　者：沈建平[1] 王昭萍[1] 霍忠明[1] 于瑞海[1] 姚托[1] 王康[1] 

机构地区：[1]中国海洋大学海水养殖教育部重点实验室,山东青岛266003

出　　处：《海洋湖沼通报》2013年第3期67-72,共6页Transactions of Oceanology and Limnology

基　　金：国家自然科学基金项目(33172403);国家重点基础研究发展规划项目(2010CB126400)资助

摘　　要：本研究采用荧光显微技术对香港巨牡蛎(Crassostrea hongkongensis)♀×近江牡蛎(C.ariakensis)♂的受精过程以及受精卵的早期发育进行了观察。结果表明:杂交组的受精率为35.3%±1.86%,大部分受精卵可以正常发育,但其发育速度相对种内受精组较慢,实验已获得了成活的2龄个体。本研究证明了香港巨牡蛎♀×近江牡蛎♂种间杂交的可行性。The fertilization and early embryogenesis of Crassostrea hongkongensis ♀× C. ariakensis ♂ was performed in this study. A consecutive study was conducted on the eggs produced from the fertili zation and early embryogenesis with a fluorescent microscope in the hybridization between C. hongkon- gensis ♀ and C. ariakensis♂. Results indicated that the rate of crossing fertilization was about 35.3± 1.86%, with most crossing developing normally, however, the rate of early embryogenetic develop- ment of C. hongleongensis♀× C. ariakensis♂ was slower compared to the C. hongkongensis and C. ariakensis control groups. This study primarily suggested the possibility of interspecific hybridization between the C. hongteongensis ♀×C. ariakensis ♂.

关 键 词：香港巨牡蛎 近江牡蛎 受精 杂交育种 早期胚胎发育 

分 类 号：S968[农业科学—水产养殖]