维药毛菊苣萃取物对肝纤维化大鼠的保护作用  

Protective effects of Cichorium glandulosum Boiss extracts on liver fibrosis in rats

作　　者：秦冬梅[1] 文志萍[1] 王新春[2] 曹文江[2] 陈文[1] 

机构地区：[1]石河子大学药学院,新疆石河子832002 [2]石河子大学第一附属医院,新疆石河子832000

出　　处：《中国医院药学杂志》2013年第18期1480-1483,共4页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金(编号:81060255);国家"重大新药创制"科技重大专项(编号:2011ZX09401-007)

摘　　要：目的:研究维药毛菊苣萃取物(CG-Ⅰ,CG-Ⅱ,CG-Ⅲ)对硫代乙酰胺(TAA)诱导肝纤维化大鼠的保护作用。方法:灌胃毛菊苣(CG-Ⅰ,CG-Ⅱ,CG-Ⅲ),腹腔注射硫代乙酰胺(TAA)造肝纤维化模型,考察肝脏系数、脾脏系数,血清谷草转氨酶(AST)、谷丙转氨酶(ALT),肝组织中碱性磷酸酶(AKP)、乳酸脱氢酶(LDH)、谷胱甘肽过氧化物酶(GSH-PX)的水平,并观察肝脏病理组织学变化。结果:对TAA所诱导的肝纤维化动物模型,CG-Ⅰ、CG-Ⅱ和CG-Ⅲ大鼠血清中AST和ALT活性均降低,其中AST具有显著性差异(P<0.05),ALT具有极显著性差异(P<0.01);升高肝组织中GSH-Px,降低肝组织中AKP、LDH的水平,其中LDH具有显著性差异(P<0.05或P<0.01)。通过HE、Masson染色切片观察到,治疗组(CG-Ⅰ,CG-Ⅲ)的肝脏纤维化程度较模型组明显减轻。结论:维药毛菊苣萃取物对硫代乙酰胺诱导的大鼠肝纤维化具有一定的保护作用,CG-Ⅰ、CG-Ⅲ具有显著保护作用。OBJECTIVE To investigate the protective effects of Cichorium glandulosum Boiss extracts （CG-I , CG-Ⅱ and CGⅢ ） against liver fibrosis induced by thioacetamide （TAA） in rats. METItODS The experimental animal models of liver fi brosis were established by peritoneal injection of thioacetamide（TAA）, then the rats were given with consecutive gastric infu sion （CG-- I , CG- Ⅱand CG- Ⅲ in rat respectively. The liver and spleen indexes ; aspartate amiontransferase （ AST）, alanine aminotrans ferase （ALT） in serum; alkaline phosphatase （AKP）, lactate dehydrogenase （LDH） and lutathione peroxidase （GSH-Px） in liver homogenate; and the histopathological changes of the liver were assayed. RESULTS In the liver fibrosis animal model induced by TAA, compared with the model group, CG- I ,CG- Ⅱ and CC Ⅲgroup decreased significantly the level of serum ALT and AST （P〈0. 01 or P%0. 05）. The treatment group（CG- I , CG-Ⅱ , CG-Ⅲ ）increased the activities of GSH- Px, decreased significantly the level of liver homogenate LDH （P〈0. 01 or P〈0. 05） ; Through the observation of HE and Masson dyeing slice, the liver pathological test showed that the treatment group （CG- I ,CG-Ⅲ） alleviated the hepatic fibrosis obviously. CONCLUSION Cichorium glandulosum Boiss extracts can afford a better protective effect against thioaeetamidc （TAA） induced hepatic damage in rats. CG-I ,CG-Ⅲ has significant protective effect.

关 键 词：毛菊苣萃取物 肝纤维化 硫代乙酰胺（TAA） 药理实验 大鼠 

分 类 号：R285.5[医药卫生—中药学]