昆母汤对类风湿关节炎成纤维样滑膜细胞增殖及RANKL/骨保护素系统的影响  

Effects of Kunmu decoction on the proliferation of fibroblast-like synoviocytes and RANKL/OPG system in patients with rheumatoid arthtitis

作　　者：林昌松[1] 陈秀敏[2] 林云斌[2] 刘清平[1] 徐强[1] 关彤[1] 陈纪藩[1] 刘风震[2] 吴莹[2] 

机构地区：[1]广州中医药大学第一附属医院风湿病科,510120 [2]广州中医药大学第一临床医学院,510405

出　　处：《实用医学杂志》2013年第18期2955-2957,共3页The Journal of Practical Medicine

基　　金：国家自然科学基金面上项目(编号:81173634);广东省自然科学基金自由申请项目(编号:S2011010005660)

摘　　要：目的:观察昆母汤醇提液(KMT)对类风湿关节炎成纤维样滑膜细胞(RA-FLS)的RANKL/骨保护素系统的影响。方法:采用MTT法进行细胞增殖分析,通过RT-PCR观察RNAKL、OPGmRNA表达强度的变化及RANKL/OPG比值。结果:各干预组抑制RA-FLS增殖呈时间剂量依赖性;各干预组对高表达的RANKL mRNA有抑制作用(P<0.01),对低表达的OPG mRNA,MTX有促进作用(P<0.01),KMT中、高剂量有抑制作用(P<0.05),各干预组对RANKL/OPG比值均也有显著的抑制作用(P<0.01)。结论:昆母汤可能通过抑制RA-FLS增殖,下调RANKL/OPG的比值而抑制破骨细胞的分化和功能,发挥抗风湿作用。Objective To observe the effects of alcohol extract of Kunmu decoction （KMT） on RANKL/ OPG system of fibroblast-like synoviocytes in patients with rheumatoid arthtitis （RA-FLS）. Methods The proliferation of RA-FLS was measured by MTr method, and reahime-PCR was used to observe the expressions of RANKL and OPG mRNA and RANKL/OPG value in RA-FLS. Results The inhibiting effects on the proliferation of RA-FLS in all treatment groups were in time-and dose-dependent manner. The high-expressed RANKL mRNAs were inhibited in all treatment groups （P 〈 0.01 ）. The low-expressed OPG mRNA could be promoted by MTX （P 〈 0.01）, and be inhibited by moderate or high dosages of KMT （P 〈 0.05）. RANKL/ OPG value was significantly inhibited in all treatment groups （P 〈 0.01）. Conclusion Kunmu decoction might inhibit RA-FLS proliferation and down-regulate RANKL/OPG value so as to inhibit the differentiation and the function of osteoclasts, and finally to treat RA.

关 键 词：类风湿关节炎 昆母汤 成纤维样滑膜细胞 核因子-ΚB受体活化因子配体 骨保护素 

分 类 号：R285[医药卫生—中药学]