罗氏沼虾(Macrobrachium rosenbergii)smu-1基因的筛选、表达及功能研究  

SCREENING,EXPRESSION AND FUNCTION OF smu-1 GENES FROM MACROBRACHIUM ROSENBERGII

作　　者：钱美睿[1] 郑春静 刘军[1] 王庆强[1] 

机构地区：[1]中国计量学院生命科学学院,杭州310018 [2]宁波市海洋与渔业研究院,宁波315012

出　　处：《海洋与湖沼》2013年第4期1008-1015,共8页Oceanologia Et Limnologia Sinica

基　　金：国家863计划项目"海水养殖生物重要功能基因的发掘与研究";SS2012AA10A400号;虾类功能基因的研究开发;2012/01-2015/12

摘　　要：从热诱导下罗氏沼虾(Macrobrachium rosenbergii)中枢神经的消减文库中,筛选到一种高温应激下表达水平显著上调的smu-1基因。该基因编码的氨基酸序列与其它物种的smu-1呈现较高的同源性,该蛋白的羧基端具有WD-40 repeat结构域,该区域含有6个重复的WD基元,因此属于WD40-repeat蛋白家族。采用荧光定量PCR方法,对不同环境因子诱导下smu-1基因的表达特征进行分析,结果表明,与正常组相比,冷热休克和重金属诱导下smu-1基因的相对表达水平显著上升(P<0.01和P<0.05),而在盐胁迫smu-1表达水平却有下调的趋势(P<0.05)。进一步采用smu-1双链RNA干扰技术,深入探究了该基因的功能与高温诱导之间的相互关系。本研究在一定程度上为养殖虾类耐热性优良品种的改良和筛选提供了重要理论依据,从而为降低高温对养殖虾类造成的重大经济损失提供广泛应用前景。The differences in ultrastructure changes of esophageal sac and liver in normal and 5d hunger juvenile of Pampus argenteus was observed in scanning and transmission electron microscopy. Result showsa number of mastoids in average height of 800μm in the inner wall of esophageal sac, on which the secondary mastoids were found in normal juvenile of P. argenteus. Mastoid mucosa consisted of a stratified squamous epithelium, with fingerprint-like microridges at the surface of epithelial cellsfor playing a role of mitigation to mechanical damage of epithelial cells. The liver cells of normal fish show textures of hepatic sinus, erythrocytes, microvilli, Dieldrin sinus, bile canaliculi, and mitochondria , Hepatic cell arranged closely, containing many organelles and lipid droplets, nucleoli were large and obvious. Many mitochondria located around the nucleus, endoplasmic reticulum, and Dieldrin sinus. After starvation, the mastoids in the inner wall of esophageal sac became lowered （average height 700μm）, mastoid mucosa thinned and retreated gradually, during which sharp horny spine was exposed from the top of secondary mastoid. The structure of liver cells changed greatly after starvation： sizes of liver cells and nuclear shrank, the number of organelles reduced, mitochondria swelled, the number of rough endoplasmic reticulum and lipid droplets decreased significantly, which is a physiological defense against starvation by juvenile of P. argenteus.

关 键 词：罗氏沼虾 smu-1基因 WD40-repeat蛋白家族 高温诱导 

分 类 号：Q74[生物学—分子生物学]