不同剂量瑞舒伐他汀对不稳定斑块中MMP影响  

Effects of different dose rosuvastatin on expressions of matrix MMP-2and MMP-9 in unstable plaque in rabbits

作　　者：逄晓玲[1] 张洪颖[1] 周红[1] 朱杰[1] 

机构地区：[1]中国医科大学附属第四医院急诊急救中心,辽宁沈阳110032

出　　处：《中国公共卫生》2013年第10期1482-1484,共3页Chinese Journal of Public Health

基　　金：辽宁省自然科学基金(20092106)

摘　　要：目的建立不稳定斑块动物模型,探讨不同剂量瑞舒伐他汀对不稳定斑块中基质金属蛋白酶(MMP)-2、9及血脂影响。方法健康雄性新西兰大白兔40只,随机分为对照组、模型组及低、高剂量瑞舒伐他汀组,每组10只,饲养8周;观察实验前后总胆固醇(TC)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)、甘油三酯(TG)水平,免疫组化分析不稳定斑块中MMP-2、9表达。结果高、低剂量瑞舒伐他汀组兔血清中TC分别为(11.97±0.35)、(13.73±0.35)mmol/L,TG分别为(6.24±0.37)、(7.53±0.36)mmol/L,LDL分别为(4.28±0.23)、(4.83±0.25)mmol/L,HDL分别为(1.93±0.24)、(1.62±0.22)mmol/L;与模型组比较,高、低剂量瑞舒伐他汀组兔血清TC、TG及LDL的水平均降低(P<0.01),HDL水平均升高(P<0.05);免疫组化结果显示,高、低剂量瑞舒伐他汀组兔腹主动脉不稳定斑块中MMP-2、9表达均较模型组减少(P<0.05)。结论瑞舒伐他汀通过改善血脂水平及抑制MMP-2、9的表达稳定动脉粥样斑块。Objective To explore effects of rosuvastain on expressions of matrix metalloproteinase-2（MMP-2）and matrix metalloproteinase-9（MMP-9）in vulnerable plaque in rabbits.Methods Fourty healthy male New Zealand white rabbits were randomly divided into four groups：control group（n=10）,vulnerable plaque group（n=10）,low dose rosuvastatin group（n=10,1 mg/kg/d）and high dose rosuvastatin group（n=10,2 mg/kg/d）.Total cholesterol（TC）,low-density lipoprotein cholesterol（LDL-C）,high-density lipoprotein cholesterol（HDL-C）,and triglyceride（TG）were detected before and 1 month after the treatment,respectively.At the end of experiment,all rabbits were sacrificed and expressions of MMP-2 and MMP-9 were determined with immunohistochemical assays.Results Compared to the control group,the concentrations of TC,TG,and LDL-C significantly decreased in rosuvastain treatment groups（11.93＆plusmn;0.35 and 13.73＆plusmn;0.35 mmol/L,6.24＆plusmn;0.37 and 7.53＆plusmn;0.36 mmol/L,and 4.28＆plusmn;0.23 and 4.83＆plusmn;0.25 mmol/L for high dose and low dose group）;the concentration of HDL-C increased significantly（1.93＆plusmn;0.24 for high dose group and 1.62＆plusmn;0.22 mmol/L for low dose group）（P〈0.05 for all）.The results of immunohistochemical assays showed that the expressions of MMP-2 and MMP-9 in unstable plague of abdominal aorta were significantly decreased in high and low dose rosuvastatin treatment groups（P〈0.05 for all）compared to that of control group.Conclusion Rosuvastatin improves blood lipid levels and reduces expressions of MMP-2 and MMPv9 which might stabilize the atherosclerotic plaques in rabbits.

关 键 词：瑞舒伐他汀 动脉粥样硬化 血脂 基质金属蛋白酶(MMP) 

分 类 号：R541.4[医药卫生—心血管疾病]