新疆田旋花丛簇病植原体的分子鉴定  

Molecular Identification of Phytoplasma Strain Associated with Convolvulus arvensis Cluster Disease in Xinjiang

作　　者：石宝萍[1] 李成亮[1] 都业娟[1] 向本春[1] 

机构地区：[1]石河子大学,绿洲农作物病害防控重点实验室,新疆石河子832000

出　　处：《北方园艺》2013年第19期114-117,共4页Northern Horticulture

基　　金：国家科技支撑计划资助项目(2011BAD48B00);石河子大学自然科学创新团队资助项目(2011ZRKXTD-0205)

摘　　要：为明确新疆田旋花丛簇病植原体的分类地位,现利用植原体16SrRNA基因通用引物P1/P7和R16F2n/R16R2对新疆石河子地区表现丛簇症状的田旋花植株总DNA进行巢式PCR扩增,并对扩增片段进行克隆和测序。结果表明:田旋花丛簇病植原体16SrRNA基因片段长1 229bp(Genbank No.:KC414725),该分离物在系统发育树中与植原体16SrII组花生丛枝组(Peanut witches’broom)成员聚集在一起,与该组成员中的茄子巨芽Eggplant big bud(JX083377)植原体同源性最高,达到98%。因此确定田旋花丛簇病植原体是16SrII组成员。In order to identify the taxonomic status of phytoplasma of Convolvulus arvensis cluster disease in Xinjiang, using phytoplasma universal primers P1/P7 and R16F2n/R16R2 for 16S rRNA gene to detect phytoplasma in Convolvulus arvensis showing symptoms of cluster disease in Xinjiang Shihezi area. The target fragment was amplified from the total DNA by nested-PCR, then it was cloned and sequenced. The results showed that the 16S rRNA gene of Convolvulus arvensis cluster phytoplasma consists of 1 229 bp （Genbank No. ：KCA14725）,it clustered together with the members of phytoplasma Peanut witches＇ broom （16SrII） group, and shared a high similarity（98%）with the phytoplasma EBB-A （Eggplant big bud） belongs to 16SrII. So it was confirmed that the phytoplasma strain Convulus arvensis cluster disease was the member of phytoplasma 16SrII group.

关 键 词：田旋花 丛簇病 植原体 分子鉴定 

分 类 号：Q813.1[生物学—生物工程]