新疆展毛多根乌头中乌头碱分离及含量测定  

Separation and Quantitative Determination of Aconitine in Aconitum karakolicum var. patentipilum W. T. Wang

作　　者：闫晋晋[1] 王信隆[2] 廖国珍 杨薪正 

机构地区：[1]西安医学高等专科学校基础医学部,陕西西安710309 [2]西安交通大学医学院,陕西西安710061 [3]西安市第二人民医院,陕西西安710003 [4]西安医学高等专科学校药学系,陕西西安710309

出　　处：《安徽农业科学》2013年第19期8134-8135,共2页Journal of Anhui Agricultural Sciences

基　　金：西安医学高等专科学校自然科学研究资金资助项目(No.12072056)

摘　　要：[目的]研究展毛多根乌头有效成分乌头碱的分离及定量测定技术,为展毛多根乌头的开发利用提供试验依据。[方法]以乙醚为提取溶剂,从展毛多根乌头中分离其主要生物碱成分乌头碱(aconitine);采用改良异羟肟酸法,在波长512.6 nm波长处测定展毛多根乌头中乌头碱的含量。[结果]展毛多根乌头中乌头碱的含量为0.360 6%,平均回收率为97.40%。[结论]该定量分析方法简便、实用,显色稳定性好,为展毛多根乌头的开发利用提供了科学依据。[ Objective ] Study on the effective component separation and quantitative determination technologies of aconitine in Aconitum kara-kolicum vat. patentipilum W. T. Wang,, which lays an experimental basis for the development and utilization of A. karakolicum. [ Method ] With ethyl ether as extraction solvent, and aconitine isolated from A. karakolicum, using modified hydroxyl oxime acid method, the content of aconitine in A. karakolicum at 512.6 nm wavelength was determined. [ Result] The content of aconitine is 0. 360 6% , the average recovery rate is 97.40%. [ Conclusion] The quantitative analysis method is simple, practical, color stability, and provide scientific basis for the devel-opment and utilization of A. karakolicum.

关 键 词：展毛多根乌头(Aconitum karakolicum var.patentipilum W.T.Wang) 乌头碱 含量测定 

分 类 号：S567[农业科学—中草药栽培]