正交试验优选灵芝多糖的提取工艺  

Optimizing Extraction Technique for Ganoderma lucidum Polysaccharide by Orthogonal Test

作　　者：陈乃东[1,2] 陈琼[1] 何健[1] 

机构地区：[1]皖西学院生物与制药工程学院,安徽六安237012 [2]皖西中药与天然药物工程技术研究中心,安徽六安237012

出　　处：《安徽农业科学》2013年第19期8146-8148,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金(81274021);安徽高校省级科学研究项目(KJ2012A277;KJ20108259);六安市定向委托皖西学院市级研究重点项目(2011LWA001);皖西学院研究性学习项目(WXXYX2013055;WXXYX2013059)

摘　　要：[目的]对灵芝多糖的提取工艺进行优选。[方法]将灵芝在50℃条件下干燥恒重,过40目筛,用无水乙醇对灵芝干粉进行脱脂预处理,脱脂完毕风干后在不同的料液比、温度、提取时间的条件下进行单因素试验,并在单因素试验的基础上进行正交试验获得最佳提取工艺。[结果]灵芝多糖的最佳提取工艺为:料液比1∶20(W/V,g/ml,下同),提取时间为3 h,提取温度为90℃;在此条件下,灵芝多糖得率可达1.61%。[结论]该方法优选了灵芝多糖的提取工艺条件,为灵芝多糖的进一步开发利用提供了依据。[ Objective ] To optimize the extraction technique of Ganoderma lucidum polysaccharide. [ Method ] Ganoderma lucidum were dried under 50 ℃, after 40 sunny days, defatting pretreatment was conducted on Ganoderma lucidum dry powder with absolute ethyl alcohol. After air-drying, single-factor test was carried out under different solid-liquid ratio, temperature, extraction times. On the basis of this, orthogonal test was conducted to obtain the optimum extraction technique. [ Result] The optimum extraction technique is： solid-liquid ratio 1：20 （W/V, g/ml, the same as follows）, extraction time 3 h, temperature 90 ℃ ; under the above conditions, the yield of Ganoderma lucidum polysaccha-ride reached 1.61%. [Conclusion] The method optimized the extraction technique for Ganoderma lucidum polysaccharide, which will provide a reference for further development and utilization.

关 键 词：灵芝(GANODERMA) 多糖 正交试验 

分 类 号：S567.31[农业科学—中草药栽培]