本源微生物修复稠油污染土壤最佳生态条件优选试验  

Optimization of Ecological Conditions for Heavy Oil-contaminated Soil Remediation by an Indigenous Microorganism

作　　者：陈梅梅 马超[2] 刘光全 刘鹏 赵林[2] 汤小萌 

机构地区：[1]中国石油安全环保技术研究院,北京102206 [2]长江大学石油工程学院,湖北武汉430100

出　　处：《安徽农业科学》2013年第19期8255-8256,8284,共3页Journal of Anhui Agricultural Sciences

基　　金：中石油重大科技专项(2011E-2407)

摘　　要：[目的]探讨本源微生物修复稠油污染土壤最佳生态条件。[方法]从辽河油田稠油污染土壤中分离出本源微生物高效降解菌株,首先通过对pH、稠油浓度、营养元素、接种量等因素进行单因素试验,然后根据单因素试验结果,进一步进行正交试验得出本源微生物降解稠油污染土壤的最佳生态条件。[结果]影响微生物降解稠油污染土壤降解率因素的主次顺序依次为石油浓度>接种量>pH>KNO3>KH2PO4;微生物的最佳生态降解条件为:KNO3为0.45 g,K2HPO4为1.10 g,pH=8,接种量为2 ml,石油浓度为0.2%,此时降解率高达61.87%。[结论]该研究为稠油污染土壤的微生物修复提供了理论依据。[ Objective] The study aimed to discuss the optimal ecological conditions for heavy oil-contaminated soil remediation by an indige-nous microorganism. [ Method ] Indigenous bacteria segregated from heavy oil-contaminated soil in Liaohe River basin were used to restore the heavy oil-contaminated soil. Firstly, the effecls of pH, oil cuneentration, nutrient elements and inoeulum volume on degradation efficiency of petroleum hydrocarbons in the soil were analyzed through a single factor test, and the optimal ecological conditions for heavy oil-cuntuminated soil remediation were determined through an orthogonal test. [ Result ] Oil concentration was the most important factor influencing the degrada-tion efficiency of petroleum hydn）earhons, followed by inoeulum volume and pH. The optimal ecological conditions for heavy oil-contaminated soil remediation were as follows： dosages of KNO4 and KH2 PO4 were 0.45 and 0. 10 g respectively; pH was 8 ; inoculum wdume was 2 ml; oil concentration was 0. 2%. Under the optimal ecological conditions, the degradation efficiency reaehed 61.87%. [ Cunelusion] The research eouht provide scientific references for microbial remediation of heavy oil-cootaminated soil.

关 键 词：稠油污染土壤 微生物修复 生态条件 

分 类 号：S154.3[农业科学—土壤学] TE991.3[农业科学—农业基础科学]