人参皂苷Rg1对血管紧张素Ⅱ诱导心肌细胞肥大的影响  

Effects of Ginsenoside Rg1 on AngⅡ-induced Myocardial Hypertrophy

作　　者：余良主[1] 韩璐[1] 孟巍[1] 王柏军[1] 王帮华[1] 

机构地区：[1]湖北科技学院基础医学院,湖北咸宁437100

出　　处：《湖北科技学院学报（医学版）》2013年第4期277-280,共4页Journal of Hubei University of Science and Technology(Medical Sciences)

基　　金：湖北省教育厅科学技术研究项目(B20122804);湖北科技学院科学研究项目(BK1104;ZX1201)

摘　　要：目的探讨传统中药人参皂苷Rg1对血管紧张素Ⅱ(AngⅡ)所诱发心肌肥大的影响。方法采用酶消化法分离新生大鼠心室肌细胞,培养心肌细胞随机分为3组:正常对照组、AngⅡ组、AngⅡ+Rg1组。在接受药物处理24h后,检测心肌细胞总蛋白含量,β-MHC、TNF-α和IL-1β的mRNA表达量。结果 AngⅡ处理导致培养心肌细胞的总蛋白含量以及β-MHC mRNA表达量显著增加,提示心肌肥大的发生;人参皂苷Rg1显著抑制了AngⅡ的促心肌细胞肥大作用。同时,AngⅡ增加心肌细胞对TNF-α和IL-1βmRNA表达的效应也被人参皂苷Rg1所抑制。结论人参皂苷Rg1抑制了AngⅡ诱导的心肌细胞肥大以及心肌细胞对炎性介质TNF-α、IL-1β的释放,这可能是人参皂苷Rg1对心脏疾病具有保护作用的细胞学基础。Objective To determine the effect of ginsenoside Rg1 on angiotensin Ⅱ （ Ang Ⅱ） -induced myocardial hypertrophy. Methods Cardiomyocytes were isolated from 1 - 3 days old Sprague-Dawley rats and then randomly divided into 3 groups：normal control,Ang Ⅱgroup,Ang Ⅱ＋ Rg1 group. After 24h treatment, cardiomyocytes were collected to determine the total protein content by Lowry＇ s method, and β-myosin heavy chain （β-MHC）,TNF-α,and IL-1β mRNA expression was detected by Real-time PCR. Results Ang Ⅱ induced cardiomyocyte hypertrophy as evidenced by increased total protein content and enhanced expression of β- MHC mRNA. Ginsenoside Rg1 significantly inhibited these hypertrophic responses, suppressed Ang Ⅱ - induced increase of TNF-α and IL-1β mRNA expression in cardiomyocytes. Conclusion Ginsenoside Rg1 can inhibite cardiomyocyte hypertrophy induced by AngⅡ - and increase the release of inflammatory mediators such as TNF-α and IL-1β,which may be the cellular basis for its protective role in cardiac diseases.

关 键 词：人参皂苷RG1 血管紧张素Ⅱ 心肌细胞 TNF—α IL-1Β 

分 类 号：R284[医药卫生—中药学]