转基因甘蓝、花椰菜的抗虫检测  

LEAF TESTING OF Bt- TRANSGENIC CABBAGE (Brassica oleracea var. capitata) AND CAULIFLOWER (B. oleracea var. botrytis)FOR EVALUATING RESISTANCE TO INSECTS (cabbage moth and cabbage worm)

作　　者：钟仲贤[1] 徐悌惟[1] 卫志明[2] 徐淑平[2] 黄健秋[2] 薛红卫[2] 

机构地区：[1]上海市农业科学院园艺研究所，上海 201106 [2]中国科学院上海植物生理研究所，上海 200032

出　　处：《上海农业学报》2000年第4期10-14,共5页Acta Agriculturae Shanghai

基　　金：上海市科技兴农重点攻关项目;上海市科学技术发展基金项目

摘　　要：1997～ 1999年对甘蓝、花椰菜转Bt抗虫基因R0 代及R1代植株离体叶片进行了抗虫检测试验。结果表明 ,使用小菜蛾 1龄幼虫 ,每9cm圆形叶片接种 2 0条 ,在 2 0～ 2 2℃培养 5～6d ,以校正死亡率和叶片损害程度综合评估抗虫性 ,可取得有效的结果 ,此检测方法比较简便 ,可用于单株抗虫性的初步筛选。In vitro leaf testings of R 0 and R 1 plants from Bt-transgenic cabbage (Brassica oleracea var.capitata) and cauliflower (B.oleracea var.botrytis)for evaluating resistance to cabbage worm or cabbage moth larvae were made in 1997～1999.The results showed that good effecs might be attained through determining corrected mortality of larvae and damage degree of leaves after inoculating first-instar cabbage moth larvae on each leaf disc(9cm in diameter) and 5～6 days culture at 20～22℃. This testing method is simpler and can be used in priliminary selection of single plant resistant to cabbage moth.

关 键 词：甘蓝 花椰菜 小菜蛾 抗虫检测 

分 类 号：S635.034[农业科学—蔬菜学]