不同乙肝检测模式中Pre-S1抗原分析  

The Analysis of Pre-S1 Antigen in Different Hepatitis B Detection Mode

作　　者：孙桂军 

机构地区：[1]新矿集团莱芜中心医院,山东莱芜271103

出　　处：《中国医药指南》2013年第25期306-307,共2页Guide of China Medicine

摘　　要：目的分析不同乙肝检测模式中Pre-S1抗原与HBV-DNA的关系。方法采用酶联免疫吸附试验(ELISA),测定Pre-S1和HBcAg。用荧光定量PCR法检测HBV-DNA。比较Pre-S1抗原在各种模式中的阳性率及Pre-S1抗原与HBV-DNA的关系。结果 HBsAg(+)、HBeAb(+)、HBcAb(+)模式中,Pre-S1抗原(+)阳性率为86%,HBsAg(+)、HBeAb(+)、HBcAb(+)模式中,Pre-S1抗原(+)阳性率为40%,HBsAg(+)、HBcAb(+)模式中,Pre-S1抗原(+)阳性率为25%,结果具有统计学意义(P<0.05)。各组模式中,Pre-S1抗原阳性率与HBV-DNA比较,无显著性差异(P>0.05)。结论 Pre-S1抗原可以作为判断乙肝的补充指标,对临床实践有指导意义。Objective To analyse the different hepatitis b detection pattern in the Pre-S1 antigen and HBV-DNA . Method Used the enzyme-linked immunosorbent assay （ELISA）,and determined the Pre-S1 and HBcAg. Used lfuorescence quantitative PCR method to detect HBV-DNA. Compared the Pr-S1 antigen positive rate in all kinds of patterns and the Pre-S1 antigen and HBV-DNA. Result In HBsAg（＋）, HBeAb（＋）and HBcAb（＋） mode, the Pre-S1 antigen （＋） positive rate was 86%, the HBsAg（＋）, HBeAb（＋）and HBcAb（＋）mode, the Pre-S1 antigen （＋） positive rate was 40%, the HBsAg（＋）and HBcAb（＋）mode, the Pre-S1 antigen （＋） positive rate was 25%, the results had statistical signiifcance（P〈0.05）. Conclusion The Pre-S1 antigen can be a supplementary index, in clinical practice it can judge hepatitis b as a guidance.

关 键 词：ELISA法 荧光定量PCR法 乙肝 PRE-S1抗原 

分 类 号：R512.62[医药卫生—内科学]