hsa-miR-1908靶基因预测及生物信息学分析  

hsa-miR-1908 target genes prediction and bioinformatics analysis

作　　者：杨蕾[1,2] 季晨博[1,2] 史春梅[1,2] 陈玲[1,2] 庞玲霞[1,2] 夏黎[1,2] 郭锡熔[1,2] 倪毓辉[1,2] 

机构地区：[1]南京医科大学儿科研究所,江苏南京210029 [2]南京医科大学附属南京妇幼保健院儿童保健科,江苏南京210004

出　　处：《临床儿科杂志》2013年第9期820-824,共5页Journal of Clinical Pediatrics

基　　金：国家自然科学基金项目(No.81100618);南京市科技计划项目(No.201104013)

摘　　要：目的对hsa-miR-1908进行系统的生物信息学分析,预测其可能参与的生物学过程及信号通路,为深入研究其在人脂肪细胞分化、肥胖发生等过程中的功能与机制奠定基础。方法应用miRBase获取hsa-miR-1908的序列,并分析其特征;应用miRanda预测hsa-miR-1908的靶基因,并取预测结果及基因芯片结果的交集,进一步进行基因功能注释(Gene ontology)和生物通路富集分析(Pathway enrichment)。结果 hsa-miR-1908在各物种之间有一定保守性,其靶基因功能富集于Wnt受体信号的调控、细胞周期、细胞凋亡等生物学过程;其靶基因信号通路富集于促性腺激素释放激素信号通路、MAPK信号通路、胰岛素信号通路、细胞周期等信号转导通路及胰腺癌等疾病通路中。结论 hsa-miR-1908预测的靶基因集合富集于多个信号通路及生物学过程,与肥胖密切相关,为后续has-miR-1908在人脂肪细胞中生物学功能研究奠定基础。Objective To predict the biological process and signaling pathways in which hsa-miR-1908 might be in- volved by a series of bioinformatics analysis, so as to lay foundations and provide theoretical basis for the further studies of hsa-miR-1908 biological function in human preadipocytes. Methods The sequence ofhsa-miR-1908 was acquired from miR- Base database, and target genes of hsa-miR-1908 were predicted by miranda, and then the intersection of the results and the results of gene-chip as gene set were further analyzed by gene ontology and pathway enrichment. Results The hsa-miR-1908 had some conserved property among different species. The functions of the target genes were enriched in Wnt receptor signal- ing pathway through beta-catenin, cell cycle, cell apeptosis and other biological processes. The GnRH signaling, MAPK sig- naling, insulin signaling, cell cycle signal transduction pathways and signaling pathway in pancreatic cancer were significantly enriched. Conclusions The target genes set of hsa-miR-1908 were enriched in multiple biological process which are related with the obesity. This study provides guidance for the further study in human preadipocytes.

关 键 词：miR-1908 MIRNA 生物信息学 人脂肪细胞 肥胖 

分 类 号：R341[医药卫生—基础医学]