取食Cry2Ab蛋白后棉铃虫幼虫中肠组织的病理变化  

Pathological changes in larval midgut tissues of the cotton bollworm, Helicoverpa armigera ( Lepidoptera: Noctuidae) ,after feeding Cry2Ab

作　　者：高珍[1] 梁革梅[1] 魏纪珍[1] 张丽丽[1] 

机构地区：[1]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193

出　　处：《昆虫学报》2013年第8期934-944,共11页Acta Entomologica Sinica

基　　金：国家科技支撑计划课题(2012BAD19B05)

摘　　要：为了明确Cry2Ab杀虫蛋白的作用机制,利用透射电镜观察了棉铃虫Helicoverpa armigera(Hübner)3龄末幼虫取食含Cry2Ab蛋白(8μg/g)饲料后中肠的组织病理变化,并与分别取食含Cry1Ac蛋白(0.97μg/g)饲料和正常饲料的棉铃虫进行了比较。结果表明:棉铃虫取食Cry2Ab蛋白后中肠细胞及其细胞器均发生了明显的病变,主要表现为:中肠杯状细胞的杯腔肿胀或拉长,部分柱状细胞被杯状细胞挤压出来,微绒毛脱落,细胞核皱缩,质膜和核膜不清晰,染色质凝聚,线粒体拉伸变形,内质网肿胀断裂;并且随着取食时间的延长病变越来越明显。与取食Cry1Ac蛋白的棉铃虫相比,Cry2Ab引起棉铃虫中肠组织发生病变的速度较慢。本研究可为Cry2Ab作为转基因棉花的重要杀虫蛋白在将来更好地发挥作用提供理论依据。In order to ascertain the action mechanism of the insecticidal protein Cry2Ab, the pathological changes in the larval midgut of the cotton bollworm, Helicoverpa armigera （Hiibner） , fed on an artificial diet containing Cry2Ab protein （8 μg/g） were observed using transmission electron microscope, and the changes induced by Cryl Ae （0.97 μg/g） and the normal diet were also investigated. The results showed that the midgut cells and organelles both exhibited obvious pathological changes after H. armigera larvae fed on the artificial diet containing Cry2Ab. The main changes include that ： the lumen of the goblet cells became swollen or elongated, some columnar cells were extruded by the goblet cells, microvilli dropped off, cell nuclei shrank, the membrane of plasma and nuclear were blurred, chromatin cohered, mitochondria was stretched and deformed, and the endoplasmic reticulum swelled and broken. The pathological changes became more obvious with extension of feeding time. The pathological changes of larval midgut cells in H. armigera caused by Cry2Ab were slower than those caused by CrylAc. These results may provide the theoretical basis for better using Cry2Ab as important insecticidal protein in transgenic cotton in the future.

关 键 词：棉铃虫 杀虫蛋白 CRY2AB CRY1AC 中肠组织 病理变化 

分 类 号：Q966[生物学—昆虫学]