自噬相关基因Beclin1在大鼠深Ⅱ度烧伤皮肤中的表达  

Expression of autophagy-related gene Beclin1 on second-degree scald skin in rats

作　　者：朱绪国[1] 刘磊[1] 姜笃银[1] 于冠英[1] 李川[1] 李耀南[1] 刘振中[1] 张基勋[1] 

机构地区：[1]山东大学第二医院烧伤整形科,济南250033

出　　处：《山东大学学报（医学版）》2013年第9期60-63,共4页Journal of Shandong University:Health Sciences

基　　金：山东省优秀中青年科学家科研奖励基金(BS2012YY010);国家重点基础研究发展计划项目(2005CB522603);山东省自然科学基金青年基金(ZR2010HQ010)

摘　　要：目的观察自噬相关基因Beclin1在大鼠深Ⅱ度烧伤皮肤中的表达,初步探讨自噬与烧伤皮肤之间的关系。方法 16只雄性Wistar大鼠随机分为正常组(对照组)和烧伤组(实验组),每组各8只。实验组建立深Ⅱ度烧伤模型,烧伤后分别于1、3、7 d取材。组织学检查观察各组动物皮肤全层情况,免疫组化检测Beclin1在各组动物皮肤的表达,并行图像处理和统计学分析。结果 Beclin1基因在实验组表达阳性,对照组表达阴性,组间比较差异有统计学意义(P<0.05)。结论 Beclin1基因在Wistar大鼠烧伤皮肤中的表达呈阳性,推测自噬参与深Ⅱ度烧伤皮肤的修复过程。Objective To observe expression of autophagy-related gene Beclinlon second-degree scald tissues in rats, and to investigate the relationship of autophagy and scald tissues. Methods Sixteen Wistar rats were randomly divided into 2 groups ： the normal group （ the control group） and the scald group （ the experimental group） , 8 rats in each group. Second-degree scald models were established in experimental group, and scald tissues were obtained on day 1, 3 and 7 after scald. The rat skin in each group was evaluated by histologically examination. Expression of Beclinl in each group was detected by the immunohistochemiscal technique and conducted by image processing techniques. Statistical analyses were performed. Results Expression of Beclinl was positive in the experimental group, but negative in the control group（ P 〈 0.05 ）. Conclusion Expression of Beclinl in the experimental group was significantly higher than that in the control group, and autophagy was speculated to participate repair process of second-degree scald.

关 键 词：自噬 基因 Beclin1 大鼠 Wistar 烧伤 

分 类 号：R644[医药卫生—外科学]